Wide-field TCSPC-based fluorescence lifetime imaging (FLIM) microscopy

Klaus Suhling, Liisa M. Hirvonen, Wolfgang Becker, Stefan Smietana, Holger Netz, James Milnes, Thomas Conneely, Alix Le Marois, Ottmar Jagutzki

Research output: Chapter in Book/Conference paperConference paperpeer-review

7 Citations (Scopus)


Time-correlated single photon counting (TCSPC) is a widely used, sensitive, precise, robust and mature technique to measure photon arrival times in applications such as fluorescence spectroscopy and microscopy, light detection and ranging (lidar) and optical tomography. Wide-field TCSPC detection techniques, where the position and the arrival time of the photons are recorded simultaneously, have seen several advances in the last few years, from the microsecond to the picosecond time scale. Here, we summarise some of our recent work in this field with emphasis on microsecond resolution phosphorescence lifetime imaging (PLIM) and nanosecond fluorescence lifetime imaging (FLIM) microscopy.

Original languageEnglish
Title of host publicationAdvanced Photon Counting Techniques X
EditorsJoe C. Campbell, Mark A. Itzler
Place of PublicationUSA
PublisherSPIE - International Society for Optical Engineering
ISBN (Electronic)9781510600997
Publication statusPublished - 5 May 2016
Externally publishedYes
EventAdvanced Photon Counting Techniques X - Baltimore, United States
Duration: 20 Apr 201621 Apr 2016

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceAdvanced Photon Counting Techniques X
Country/TerritoryUnited States


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