TY - JOUR
T1 - Whole blood flow cytometry protocol for the assessment of platelet phenotype, function, and cellular interactions
AU - Yaw, Hui Ping
AU - Van den Helm, Suelyn
AU - Linden, Matthew
AU - Monagle, Paul
AU - Ignjatovic, Vera
PY - 2020/9/3
Y1 - 2020/9/3
N2 - Platelets are a key component of the hemostatic system and their roles in inflammation via interactions with leukocytes have also gained attention in recent years. Changes in platelet phenotype and function can cause bleeding and/or thrombosis and, as such, monitoring platelet-specific changes is crucial to assessing hemostasis in the clinical setting. Currently, available platelet function tests such as platelet aggregometry and thromboelastography require a large volume of blood, which is a major limitation for the pediatric population. Whole blood flow cytometric analysis of platelets is increasingly utilized in recent years, primarily due to the sensitivity of this method, but also because it only requires a small amount of blood with minimal sample manipulation. We have developed a whole blood flow cytometry methodological approach that enables the assessment of platelet phenotype, function, and their interactions with monocytes and neutrophils.
AB - Platelets are a key component of the hemostatic system and their roles in inflammation via interactions with leukocytes have also gained attention in recent years. Changes in platelet phenotype and function can cause bleeding and/or thrombosis and, as such, monitoring platelet-specific changes is crucial to assessing hemostasis in the clinical setting. Currently, available platelet function tests such as platelet aggregometry and thromboelastography require a large volume of blood, which is a major limitation for the pediatric population. Whole blood flow cytometric analysis of platelets is increasingly utilized in recent years, primarily due to the sensitivity of this method, but also because it only requires a small amount of blood with minimal sample manipulation. We have developed a whole blood flow cytometry methodological approach that enables the assessment of platelet phenotype, function, and their interactions with monocytes and neutrophils.
KW - Flow cytometry
KW - platelet function
KW - platelet-leukocyte aggregates
KW - whole blood
UR - http://www.scopus.com/inward/record.url?scp=85090222052&partnerID=8YFLogxK
U2 - 10.1080/09537104.2020.1810222
DO - 10.1080/09537104.2020.1810222
M3 - Article
C2 - 32881599
AN - SCOPUS:85090222052
JO - Platelets
JF - Platelets
SN - 0953-7104
ER -