Waterlogging Tolerance at Germination in Field Pea: Variability, Genetic Control, and Indirect Selection

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Abstract

In the Eastern Gangetic Plain of South Asia field pea (Pisum sativum L.) is often grown as a relay crop where soil waterlogging (WL) causes germination failure. To assess if selection for WL tolerance is feasible, we studied the response to WL stress at germination stage in a recombinant inbred line (RIL) population from a bi-parental cross between WL-contrasting parents and in a diversity panel to identify extreme phenotypes, understand the genetics of WL tolerance and find traits for possible use in indirect selection. The RIL population and the diversity panel were screened to test the ability of germination under both waterlogged and drained soils. A total of 50, most WL tolerant and sensitive, genotypes from each of both the RIL and the diversity panel were further evaluated to assay testa integrity/leakage in CaSO4 solution. Morphological characterization of both populations was undertaken. A wide range of variation in the ability to germination in waterlogged soil was observed in the RIL population (6-93%) and the diversity panel (5-100%) with a high broad-sense heritability (H-2 > 85%). The variation was continuously distributed indicating polygenic control. Most genotypes with a dark colored testa (90%) were WL tolerant, whereas those with a light colored testa were all WL sensitive in both the RIL population and diversity panel. Testa integrity, measured by electrical conductivity of the leakage solute, was strongly associated with WL tolerance in the RIL population (r(G) = -1.00) and the diversity panel (r(G) = -0.90). Therefore, testa integrity can be effectively used in indirect selection for WL tolerance. Response to selection for WL tolerance at germination is confidently predicted enabling the adaptation of the ancient model pea to extreme precipitation events at germination.

Original languageEnglish
Article number953
Number of pages11
JournalFrontiers in Plant Science
Volume10
DOIs
Publication statusPublished - 30 Jul 2019

Cite this

@article{4a437ec0359040c5a04f73be54566341,
title = "Waterlogging Tolerance at Germination in Field Pea: Variability, Genetic Control, and Indirect Selection",
abstract = "In the Eastern Gangetic Plain of South Asia field pea (Pisum sativum L.) is often grown as a relay crop where soil waterlogging (WL) causes germination failure. To assess if selection for WL tolerance is feasible, we studied the response to WL stress at germination stage in a recombinant inbred line (RIL) population from a bi-parental cross between WL-contrasting parents and in a diversity panel to identify extreme phenotypes, understand the genetics of WL tolerance and find traits for possible use in indirect selection. The RIL population and the diversity panel were screened to test the ability of germination under both waterlogged and drained soils. A total of 50, most WL tolerant and sensitive, genotypes from each of both the RIL and the diversity panel were further evaluated to assay testa integrity/leakage in CaSO4 solution. Morphological characterization of both populations was undertaken. A wide range of variation in the ability to germination in waterlogged soil was observed in the RIL population (6-93{\%}) and the diversity panel (5-100{\%}) with a high broad-sense heritability (H-2 > 85{\%}). The variation was continuously distributed indicating polygenic control. Most genotypes with a dark colored testa (90{\%}) were WL tolerant, whereas those with a light colored testa were all WL sensitive in both the RIL population and diversity panel. Testa integrity, measured by electrical conductivity of the leakage solute, was strongly associated with WL tolerance in the RIL population (r(G) = -1.00) and the diversity panel (r(G) = -0.90). Therefore, testa integrity can be effectively used in indirect selection for WL tolerance. Response to selection for WL tolerance at germination is confidently predicted enabling the adaptation of the ancient model pea to extreme precipitation events at germination.",
keywords = "germination, waterlogging tolerance, indirect selection, secondary traits, Pisum sp., SEASON GRAIN LEGUMES, FLOODING TOLERANCE, ANTIOXIDANT ACTIVITY, SEED-GERMINATION, WATER, TESTA, CONDUCTIVITY, HERITABILITY, METABOLISM, IMBIBITION",
author = "Zaman, {Md Shahin Uz} and Malik, {Al Imran} and Parwinder Kaur and Ribalta, {Federico Martin} and William Erskine",
year = "2019",
month = "7",
day = "30",
doi = "10.3389/fpls.2019.00953",
language = "English",
volume = "10",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media SA",

}

TY - JOUR

T1 - Waterlogging Tolerance at Germination in Field Pea

T2 - Variability, Genetic Control, and Indirect Selection

AU - Zaman, Md Shahin Uz

AU - Malik, Al Imran

AU - Kaur, Parwinder

AU - Ribalta, Federico Martin

AU - Erskine, William

PY - 2019/7/30

Y1 - 2019/7/30

N2 - In the Eastern Gangetic Plain of South Asia field pea (Pisum sativum L.) is often grown as a relay crop where soil waterlogging (WL) causes germination failure. To assess if selection for WL tolerance is feasible, we studied the response to WL stress at germination stage in a recombinant inbred line (RIL) population from a bi-parental cross between WL-contrasting parents and in a diversity panel to identify extreme phenotypes, understand the genetics of WL tolerance and find traits for possible use in indirect selection. The RIL population and the diversity panel were screened to test the ability of germination under both waterlogged and drained soils. A total of 50, most WL tolerant and sensitive, genotypes from each of both the RIL and the diversity panel were further evaluated to assay testa integrity/leakage in CaSO4 solution. Morphological characterization of both populations was undertaken. A wide range of variation in the ability to germination in waterlogged soil was observed in the RIL population (6-93%) and the diversity panel (5-100%) with a high broad-sense heritability (H-2 > 85%). The variation was continuously distributed indicating polygenic control. Most genotypes with a dark colored testa (90%) were WL tolerant, whereas those with a light colored testa were all WL sensitive in both the RIL population and diversity panel. Testa integrity, measured by electrical conductivity of the leakage solute, was strongly associated with WL tolerance in the RIL population (r(G) = -1.00) and the diversity panel (r(G) = -0.90). Therefore, testa integrity can be effectively used in indirect selection for WL tolerance. Response to selection for WL tolerance at germination is confidently predicted enabling the adaptation of the ancient model pea to extreme precipitation events at germination.

AB - In the Eastern Gangetic Plain of South Asia field pea (Pisum sativum L.) is often grown as a relay crop where soil waterlogging (WL) causes germination failure. To assess if selection for WL tolerance is feasible, we studied the response to WL stress at germination stage in a recombinant inbred line (RIL) population from a bi-parental cross between WL-contrasting parents and in a diversity panel to identify extreme phenotypes, understand the genetics of WL tolerance and find traits for possible use in indirect selection. The RIL population and the diversity panel were screened to test the ability of germination under both waterlogged and drained soils. A total of 50, most WL tolerant and sensitive, genotypes from each of both the RIL and the diversity panel were further evaluated to assay testa integrity/leakage in CaSO4 solution. Morphological characterization of both populations was undertaken. A wide range of variation in the ability to germination in waterlogged soil was observed in the RIL population (6-93%) and the diversity panel (5-100%) with a high broad-sense heritability (H-2 > 85%). The variation was continuously distributed indicating polygenic control. Most genotypes with a dark colored testa (90%) were WL tolerant, whereas those with a light colored testa were all WL sensitive in both the RIL population and diversity panel. Testa integrity, measured by electrical conductivity of the leakage solute, was strongly associated with WL tolerance in the RIL population (r(G) = -1.00) and the diversity panel (r(G) = -0.90). Therefore, testa integrity can be effectively used in indirect selection for WL tolerance. Response to selection for WL tolerance at germination is confidently predicted enabling the adaptation of the ancient model pea to extreme precipitation events at germination.

KW - germination

KW - waterlogging tolerance

KW - indirect selection

KW - secondary traits

KW - Pisum sp.

KW - SEASON GRAIN LEGUMES

KW - FLOODING TOLERANCE

KW - ANTIOXIDANT ACTIVITY

KW - SEED-GERMINATION

KW - WATER

KW - TESTA

KW - CONDUCTIVITY

KW - HERITABILITY

KW - METABOLISM

KW - IMBIBITION

U2 - 10.3389/fpls.2019.00953

DO - 10.3389/fpls.2019.00953

M3 - Article

VL - 10

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 953

ER -