This study compared VO2, heart rate (HR) and electromyographic (iEMG) responses to speeds above the velocity associated with VO2max (v-VO2max). Eight male, middle-distance runners performed a graded exercise test to determine VO2max and v-VO2max and runs to fatigue at 100% and 110%v-VO2max. Breath-by-breath VO2 and HR were continuously recorded; lactate [La-] measured pre- and post-run and iEMG measures of rectus femoris (RF) and vastus lateralis were recorded during the first and last 20 s of each run. Analysis indicated longer time to fatigue in the 100% v-VO2max run with no differences between conditions for VO2 or HR amplitudes or post-run [La-] (p > 0.05). There were significantly faster tau values (p <0.05) in the 110% condition in VO2 and HR. No significant correlations were observed between VO2 or HR tau values and time to fatigue. RF iEMG was significantly larger in 110% compared to 100% run in the first 20s (p <0.05). While no association between treadmill performance and VO2 response was evident, faster running speeds resulted in faster VO2 and HR responses, with no difference in amplitude or % VO2max attained. This may potentially be as a result of an increased muscle fibre recruitment stimulus during the faster running velocity resulting in faster cardiodynamic responses.