Vitamin D supplementation of initially vitamin D-deficient mice diminishes lung inflammation with limited effects on pulmonary epithelial integrity

Shelley Gorman, Alysia G. Buckley, Kak Ming Ling, Luke J. Berry, Vanessa S. Fear, Stephen M. Stick, Alexander N. Larcombe, Anthony Kicic, Prue H. Hart

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In disease settings, vitamin D may be important for maintaining optimal lung epithelial integrity and suppressing inflammation, but less is known of its effects prior to disease onset. Female BALB/c dams were fed a vitamin D3-supplemented (2280 IU/kg, VitD+) or nonsupplemented (0 IU/kg, VitD) diet from 3 weeks of age, and mated at 8 weeks of age. Male offspring were fed the same diet as their mother. Some offspring initially fed the VitD diet were switched to a VitD+ diet from 8 weeks of age (VitD−/+). At 12 weeks of age, signs of low-level inflammation were observed in the bronchoalveolar lavage fluid (BALF) of VitD mice (more macrophages and neutrophils), which were suppressed by subsequent supplementation with vitamin D3. There was no difference in the level of expression of the tight junction proteins occludin or claudin-1 in lung epithelial cells of VitD+ mice compared to VitD mice; however, claudin-1 levels were reduced when initially vitamin D-deficient mice were fed the vitamin D3-containing diet (VitD−/+). Reduced total IgM levels were detected in BALF and serum of VitD−/+ mice compared to VitD+ mice. Lung mRNA levels of the vitamin D receptor (VDR) were greatest in VitD−/+ mice. Total IgG levels in BALF were greater in mice fed the vitamin D3-containing diet, which may be explained by increased activation of B cells in airway-draining lymph nodes. These findings suggest that supplementation of initially vitamin D-deficient mice with vitamin D3 suppresses signs of lung inflammation but has limited effects on the epithelial integrity of the lungs.

Original languageEnglish
Article numbere13371
Pages (from-to)1-12
JournalPhysiological Reports
Volume5
Issue number15
DOIs
Publication statusPublished - 1 Aug 2017

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Vitamin D
Pneumonia
Cholecalciferol
Lung
Diet
Bronchoalveolar Lavage Fluid
Claudin-1
Inflammation
Occludin
Tight Junction Proteins
Calcitriol Receptors
Immunoglobulin M
Neutrophils
B-Lymphocytes
Immunoglobulin G
Lymph Nodes
Epithelial Cells
Macrophages
Messenger RNA
Serum

Cite this

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title = "Vitamin D supplementation of initially vitamin D-deficient mice diminishes lung inflammation with limited effects on pulmonary epithelial integrity",
abstract = "In disease settings, vitamin D may be important for maintaining optimal lung epithelial integrity and suppressing inflammation, but less is known of its effects prior to disease onset. Female BALB/c dams were fed a vitamin D3-supplemented (2280 IU/kg, VitD+) or nonsupplemented (0 IU/kg, VitD−) diet from 3 weeks of age, and mated at 8 weeks of age. Male offspring were fed the same diet as their mother. Some offspring initially fed the VitD− diet were switched to a VitD+ diet from 8 weeks of age (VitD−/+). At 12 weeks of age, signs of low-level inflammation were observed in the bronchoalveolar lavage fluid (BALF) of VitD− mice (more macrophages and neutrophils), which were suppressed by subsequent supplementation with vitamin D3. There was no difference in the level of expression of the tight junction proteins occludin or claudin-1 in lung epithelial cells of VitD+ mice compared to VitD− mice; however, claudin-1 levels were reduced when initially vitamin D-deficient mice were fed the vitamin D3-containing diet (VitD−/+). Reduced total IgM levels were detected in BALF and serum of VitD−/+ mice compared to VitD+ mice. Lung mRNA levels of the vitamin D receptor (VDR) were greatest in VitD−/+ mice. Total IgG levels in BALF were greater in mice fed the vitamin D3-containing diet, which may be explained by increased activation of B cells in airway-draining lymph nodes. These findings suggest that supplementation of initially vitamin D-deficient mice with vitamin D3 suppresses signs of lung inflammation but has limited effects on the epithelial integrity of the lungs.",
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author = "Shelley Gorman and Buckley, {Alysia G.} and Ling, {Kak Ming} and Berry, {Luke J.} and Fear, {Vanessa S.} and Stick, {Stephen M.} and Larcombe, {Alexander N.} and Anthony Kicic and Hart, {Prue H.}",
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Vitamin D supplementation of initially vitamin D-deficient mice diminishes lung inflammation with limited effects on pulmonary epithelial integrity. / Gorman, Shelley; Buckley, Alysia G.; Ling, Kak Ming; Berry, Luke J.; Fear, Vanessa S.; Stick, Stephen M.; Larcombe, Alexander N.; Kicic, Anthony; Hart, Prue H.

In: Physiological Reports, Vol. 5, No. 15, e13371, 01.08.2017, p. 1-12.

Research output: Contribution to journalArticle

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T1 - Vitamin D supplementation of initially vitamin D-deficient mice diminishes lung inflammation with limited effects on pulmonary epithelial integrity

AU - Gorman, Shelley

AU - Buckley, Alysia G.

AU - Ling, Kak Ming

AU - Berry, Luke J.

AU - Fear, Vanessa S.

AU - Stick, Stephen M.

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AU - Kicic, Anthony

AU - Hart, Prue H.

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