Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner

M. Roggenbuck, Denise Anderson, K.K. Barfod, M. Feelisch, Sian Geldenhuys, S.J. Sørensen, Clare E. Weeden, Prudence H. Hart, Shelley Gorman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

© 2016 The Author(s).Background: Vitamin D is under scrutiny as a potential regulator of the development of respiratory diseases characterised by chronic lung inflammation, including asthma and chronic obstructive pulmonary disease. It has anti-inflammatory effects; however, knowledge around the relationship between dietary vitamin D, inflammation and the microbiome in the lungs is limited. In our previous studies, we observed more inflammatory cells in the bronchoalveolar lavage fluid and increased bacterial load in the lungs of vitamin D-deficient male mice with allergic airway disease, suggesting that vitamin D might modulate the lung microbiome. In the current study, we examined in more depth the effects of vitamin D deficiency initiated early in life, and subsequent supplementation with dietary vitamin D on the composition of the lung microbiome and the extent of respiratory inflammation. Methods: BALB/c dams were fed a vitamin D-supplemented or -deficient diet throughout gestation and lactation, with offspring continued on this diet post-natally. Some initially deficient offspring were fed a supplemented diet from 8 weeks of age. The lungs of naïve adult male and female offspring were compared prior to the induction of allergic airway disease. In further experiments, offspring were sensitised and boosted with the experimental allergen, ovalbumin (OVA), and T helper type 2-skewing adjuvant, aluminium hydroxide, followed by a single respiratory challenge with OVA. Results: In mice fed a vitamin D-containing diet throughout life, a sex difference in the lung microbial community was observed, with increased levels of an Acinetobacter operational taxonomic unit (OTU) in female lungs compared to male lungs. This effect was not observed in vitamin D-deficient mice or initially deficient mice supplemented with vitamin D from early adulthood. In addition, serum 25-hydroxyvitamin D levels inversely correlated with total bacterial OTUs, and Pseudomonas OTUs in the lungs. Increased levels of the antimicrobial murine ß-
Original languageEnglish
Pages (from-to)116
Number of pages116
JournalRespiratory Research
Volume17
Issue number1
DOIs
Publication statusPublished - 2016

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Microbiota
Vitamin D
Lung
Diet
Ovalbumin
Inflammation
Aluminum Hydroxide
Acinetobacter
Vitamin D Deficiency
Bacterial Load
Bronchoalveolar Lavage Fluid
Dietary Supplements
Pseudomonas
Lactation
Sex Characteristics
Allergens
Chronic Obstructive Pulmonary Disease
Pneumonia
Chronic Disease
Anti-Inflammatory Agents

Cite this

Roggenbuck, M. ; Anderson, Denise ; Barfod, K.K. ; Feelisch, M. ; Geldenhuys, Sian ; Sørensen, S.J. ; Weeden, Clare E. ; Hart, Prudence H. ; Gorman, Shelley. / Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner. In: Respiratory Research. 2016 ; Vol. 17, No. 1. pp. 116.
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abstract = "{\circledC} 2016 The Author(s).Background: Vitamin D is under scrutiny as a potential regulator of the development of respiratory diseases characterised by chronic lung inflammation, including asthma and chronic obstructive pulmonary disease. It has anti-inflammatory effects; however, knowledge around the relationship between dietary vitamin D, inflammation and the microbiome in the lungs is limited. In our previous studies, we observed more inflammatory cells in the bronchoalveolar lavage fluid and increased bacterial load in the lungs of vitamin D-deficient male mice with allergic airway disease, suggesting that vitamin D might modulate the lung microbiome. In the current study, we examined in more depth the effects of vitamin D deficiency initiated early in life, and subsequent supplementation with dietary vitamin D on the composition of the lung microbiome and the extent of respiratory inflammation. Methods: BALB/c dams were fed a vitamin D-supplemented or -deficient diet throughout gestation and lactation, with offspring continued on this diet post-natally. Some initially deficient offspring were fed a supplemented diet from 8 weeks of age. The lungs of na{\"i}ve adult male and female offspring were compared prior to the induction of allergic airway disease. In further experiments, offspring were sensitised and boosted with the experimental allergen, ovalbumin (OVA), and T helper type 2-skewing adjuvant, aluminium hydroxide, followed by a single respiratory challenge with OVA. Results: In mice fed a vitamin D-containing diet throughout life, a sex difference in the lung microbial community was observed, with increased levels of an Acinetobacter operational taxonomic unit (OTU) in female lungs compared to male lungs. This effect was not observed in vitamin D-deficient mice or initially deficient mice supplemented with vitamin D from early adulthood. In addition, serum 25-hydroxyvitamin D levels inversely correlated with total bacterial OTUs, and Pseudomonas OTUs in the lungs. Increased levels of the antimicrobial murine {\ss}-",
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Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner. / Roggenbuck, M.; Anderson, Denise; Barfod, K.K.; Feelisch, M.; Geldenhuys, Sian; Sørensen, S.J.; Weeden, Clare E.; Hart, Prudence H.; Gorman, Shelley.

In: Respiratory Research, Vol. 17, No. 1, 2016, p. 116.

Research output: Contribution to journalArticle

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T1 - Vitamin D and allergic airway disease shape the murine lung microbiome in a sex-specific manner

AU - Roggenbuck, M.

AU - Anderson, Denise

AU - Barfod, K.K.

AU - Feelisch, M.

AU - Geldenhuys, Sian

AU - Sørensen, S.J.

AU - Weeden, Clare E.

AU - Hart, Prudence H.

AU - Gorman, Shelley

PY - 2016

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AB - © 2016 The Author(s).Background: Vitamin D is under scrutiny as a potential regulator of the development of respiratory diseases characterised by chronic lung inflammation, including asthma and chronic obstructive pulmonary disease. It has anti-inflammatory effects; however, knowledge around the relationship between dietary vitamin D, inflammation and the microbiome in the lungs is limited. In our previous studies, we observed more inflammatory cells in the bronchoalveolar lavage fluid and increased bacterial load in the lungs of vitamin D-deficient male mice with allergic airway disease, suggesting that vitamin D might modulate the lung microbiome. In the current study, we examined in more depth the effects of vitamin D deficiency initiated early in life, and subsequent supplementation with dietary vitamin D on the composition of the lung microbiome and the extent of respiratory inflammation. Methods: BALB/c dams were fed a vitamin D-supplemented or -deficient diet throughout gestation and lactation, with offspring continued on this diet post-natally. Some initially deficient offspring were fed a supplemented diet from 8 weeks of age. The lungs of naïve adult male and female offspring were compared prior to the induction of allergic airway disease. In further experiments, offspring were sensitised and boosted with the experimental allergen, ovalbumin (OVA), and T helper type 2-skewing adjuvant, aluminium hydroxide, followed by a single respiratory challenge with OVA. Results: In mice fed a vitamin D-containing diet throughout life, a sex difference in the lung microbial community was observed, with increased levels of an Acinetobacter operational taxonomic unit (OTU) in female lungs compared to male lungs. This effect was not observed in vitamin D-deficient mice or initially deficient mice supplemented with vitamin D from early adulthood. In addition, serum 25-hydroxyvitamin D levels inversely correlated with total bacterial OTUs, and Pseudomonas OTUs in the lungs. Increased levels of the antimicrobial murine ß-

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DO - 10.1186/s12931-016-0435-3

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SP - 116

JO - Respiratory Research

JF - Respiratory Research

SN - 1465-9921

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