Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)

Y. Chew, A.J. Holmes, John Cliff

Research output: Chapter in Book/Conference paperChapter

3 Citations (Scopus)

Abstract

NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS. © 2014 Springer Science+Business Media, LLC.
Original languageEnglish
Title of host publicationEnvironmental Microbiology: Methods and Protocols
EditorsIan T. Paulsen, Andrew J. Holmes
PublisherHumana Press
Pages133-146
Volume1096
ISBN (Print)9781627037129
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Microbiology

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