Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)

Y. Chew; A.J. Holmes; John Cliff

doi:10.1007/978-1-62703-712-9_11

Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)

Y. Chew, A.J. Holmes, John Cliff

Centre for Microscopy, Characterisation & Analysis

Research output: Chapter in Book/Conference paper › Chapter

3 Citations (Scopus)

Abstract

NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS. © 2014 Springer Science+Business Media, LLC.

Original language	English
Title of host publication	Environmental Microbiology: Methods and Protocols
Editors	Ian T. Paulsen, Andrew J. Holmes
Publisher	Humana Press
Pages	133-146
Volume	1096
ISBN (Print)	9781627037129
DOIs	https://doi.org/10.1007/978-1-62703-712-9_11
Publication status	Published - 2014

Publication series

Name	Methods in Microbiology

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10.1007/978-1-62703-712-9_11

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title = "Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)",

abstract = "NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS. {\textcopyright} 2014 Springer Science+Business Media, LLC.",

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Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS). / Chew, Y.; Holmes, A.J.; Cliff, John.

Environmental Microbiology: Methods and Protocols. ed. / Ian T. Paulsen; Andrew J. Holmes. Vol. 1096 Humana Press, 2014. p. 133-146 (Methods in Microbiology).

Research output: Chapter in Book/Conference paper › Chapter

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T1 - Visualization of metabolic properties of bacterial cells using nanoscale secondary ion mass spectrometry (NanoSIMS)

AU - Chew, Y.

AU - Holmes, A.J.

AU - Cliff, John

PY - 2014

Y1 - 2014

N2 - NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS. © 2014 Springer Science+Business Media, LLC.

AB - NanoSIMS combines high-resolution imaging and mass spectrometry with simultaneous collection of up to seven different masses, providing an invaluable technique for determining the isotopic and elemental composition in microscopic target samples. It has been used in varying fields, from studying the elemental composition of mineral samples to tracking cell uptake of isotope-labelled substrates. In combination with in situ hybridization techniques, NanoSIMS offers a powerful method of linking metabolic capacity to phylogenetic identity in cell samples. Here, we describe methods and considerations for microbial sample preparation, visualization, and analysis using NanoSIMS. © 2014 Springer Science+Business Media, LLC.

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DO - 10.1007/978-1-62703-712-9_11

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T3 - Methods in Microbiology

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BT - Environmental Microbiology: Methods and Protocols

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A2 - Holmes, Andrew J.

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