Viral vectored immunocontraception: Screening of multiple fertility antigens using murine cytomegalovirus as a vaccine vector

Alec Redwood, N.L. Harvey, Megan Lloyd, Malcolm Lawson, C.M. Hardy, Geoffrey Shellam

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Mouse cytomegalovirus (MCMV) has previously been used as a vaccine vector for viral vectored immunocontraception (VVIC). MCMV expressing murine zona pellucida 3 (mZP3) induces long term infertility in up to 100% of female, BALB/c mice following a single inoculation. Whilst a large number of antigens have been investigated as potential immunocontraceptive vaccines, it has been difficult to compare these antigens as few studies have used identical approaches or even animal species. Here a range of protein and polyepitope antigens, all expressed by MCMV, were tested for the ability to sterilise female mice. The antigens tested were bone morphogenic protein 15 (BMP15), oviduct glycoprotein (OGP) and ubiquitin-tagged mZP3. In addition, four polyepitope constructs that contain rodent or mouse specific epitopes were tested. This study found that when expressed by an MCMV vector, only full-length mZP3 or ubiquitin-tagged mZP3 induced infertility in female mice. BNIP 15 and OGP had no effect. Of the four polyepitopes tested, one had a partial effect on fertility. These data indicate that while MCMV is an effective vector for VVIC, the antigen used needs to be tested empirically. The partial infertility seen in mice infected with one of the polyepitope vaccines is a promising finding suggesting that it may be possible to combine a species specific virus with a species specific antigen for use as a disseminating mouse control agent. (c) 2006 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)698-708
JournalVaccine
Volume25
Issue number4
DOIs
Publication statusPublished - 2007

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Immunologic Contraception
immunocontraception
Muromegalovirus
vector vaccines
Cytomegalovirus
Fertility
Murid herpesvirus 1
Zona Pellucida
Vaccines
screening
antigens
Antigens
mice
zona pellucida
Oviducts
Ubiquitin
Infertility
Glycoproteins
Viral Vaccines
Female Infertility

Cite this

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title = "Viral vectored immunocontraception: Screening of multiple fertility antigens using murine cytomegalovirus as a vaccine vector",
abstract = "Mouse cytomegalovirus (MCMV) has previously been used as a vaccine vector for viral vectored immunocontraception (VVIC). MCMV expressing murine zona pellucida 3 (mZP3) induces long term infertility in up to 100{\%} of female, BALB/c mice following a single inoculation. Whilst a large number of antigens have been investigated as potential immunocontraceptive vaccines, it has been difficult to compare these antigens as few studies have used identical approaches or even animal species. Here a range of protein and polyepitope antigens, all expressed by MCMV, were tested for the ability to sterilise female mice. The antigens tested were bone morphogenic protein 15 (BMP15), oviduct glycoprotein (OGP) and ubiquitin-tagged mZP3. In addition, four polyepitope constructs that contain rodent or mouse specific epitopes were tested. This study found that when expressed by an MCMV vector, only full-length mZP3 or ubiquitin-tagged mZP3 induced infertility in female mice. BNIP 15 and OGP had no effect. Of the four polyepitopes tested, one had a partial effect on fertility. These data indicate that while MCMV is an effective vector for VVIC, the antigen used needs to be tested empirically. The partial infertility seen in mice infected with one of the polyepitope vaccines is a promising finding suggesting that it may be possible to combine a species specific virus with a species specific antigen for use as a disseminating mouse control agent. (c) 2006 Elsevier Ltd. All rights reserved.",
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Viral vectored immunocontraception: Screening of multiple fertility antigens using murine cytomegalovirus as a vaccine vector. / Redwood, Alec; Harvey, N.L.; Lloyd, Megan; Lawson, Malcolm; Hardy, C.M.; Shellam, Geoffrey.

In: Vaccine, Vol. 25, No. 4, 2007, p. 698-708.

Research output: Contribution to journalArticle

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AU - Harvey, N.L.

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AU - Shellam, Geoffrey

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