Vegetative and reproductive growth of salt-stressed chickpea are carbon-limited: Sucrose infusion at the reproductive stage improves salt tolerance

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Abstract

Reproductive processes of chickpea (Cicer arietinum L.) are particularly sensitive to salinity. We tested whether limited photoassimilate availability contributes to reproductive failure in salt-stressed chickpea. Rupali, a salt-sensitive genotype, was grown in aerated nutrient solution, either with non-saline (control) or 30mM NaCl treatment. At flowering, stems were either infused with sucrose solution (0.44M), water only or maintained without any infusion, for 75 d. The sucrose and water infusion treatments of non-saline plants had no effect on growth or yield, but photosynthesis declined in response to sucrose infusion. Salt stress reduced photosynthesis, decreased tissue sugars by 22-47%, and vegetative and reproductive growth were severely impaired. Sucrose infusion of salt-treated plants increased total sugars in stems, leaves and developing pods, to levels similar to those of non-saline plants. In salt-stressed plants, sucrose infusion increased dry mass (2.6-fold), pod numbers (3.8-fold), seed numbers (6.5-fold) and seed yield (10.4-fold), yet vegetative growth and reproductive failure were not rescued completely by sucrose infusion. Sucrose infusion partly rescued reproductive failure in chickpea by increasing vegetative growth enabling more flower production and by providing sucrose for pod and seed growth. We conclude that insuffcient assimilate availability limits yield in salt-stressed chickpea.

Original languageEnglish
Pages (from-to)2001-2011
Number of pages11
JournalJournal of Experimental Botany
Volume68
Issue number8
DOIs
Publication statusPublished - 1 Apr 2017

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Salt-Tolerance
Cicer
salt tolerance
Sucrose
Carbon
Salts
sucrose
salts
carbon
Growth
pods
Seeds
Photosynthesis
vegetative growth
photosynthesis
sugars
stems
Water Purification
Salinity
Cicer arietinum

Cite this

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title = "Vegetative and reproductive growth of salt-stressed chickpea are carbon-limited: Sucrose infusion at the reproductive stage improves salt tolerance",
abstract = "Reproductive processes of chickpea (Cicer arietinum L.) are particularly sensitive to salinity. We tested whether limited photoassimilate availability contributes to reproductive failure in salt-stressed chickpea. Rupali, a salt-sensitive genotype, was grown in aerated nutrient solution, either with non-saline (control) or 30mM NaCl treatment. At flowering, stems were either infused with sucrose solution (0.44M), water only or maintained without any infusion, for 75 d. The sucrose and water infusion treatments of non-saline plants had no effect on growth or yield, but photosynthesis declined in response to sucrose infusion. Salt stress reduced photosynthesis, decreased tissue sugars by 22-47{\%}, and vegetative and reproductive growth were severely impaired. Sucrose infusion of salt-treated plants increased total sugars in stems, leaves and developing pods, to levels similar to those of non-saline plants. In salt-stressed plants, sucrose infusion increased dry mass (2.6-fold), pod numbers (3.8-fold), seed numbers (6.5-fold) and seed yield (10.4-fold), yet vegetative growth and reproductive failure were not rescued completely by sucrose infusion. Sucrose infusion partly rescued reproductive failure in chickpea by increasing vegetative growth enabling more flower production and by providing sucrose for pod and seed growth. We conclude that insuffcient assimilate availability limits yield in salt-stressed chickpea.",
keywords = "Chickpea (Cicer arietinum L.), Flowering, Photosynthate supply and demand, Photosynthesis, Plant sucrose infusion, Podding, Salinity stress, Seed growth, Tissue ions, Tissue sugars",
author = "Khan, {Hammad A.} and Siddique, {Kadambot H.M.} and Colmer, {Timothy D.}",
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TY - JOUR

T1 - Vegetative and reproductive growth of salt-stressed chickpea are carbon-limited

T2 - Sucrose infusion at the reproductive stage improves salt tolerance

AU - Khan, Hammad A.

AU - Siddique, Kadambot H.M.

AU - Colmer, Timothy D.

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Reproductive processes of chickpea (Cicer arietinum L.) are particularly sensitive to salinity. We tested whether limited photoassimilate availability contributes to reproductive failure in salt-stressed chickpea. Rupali, a salt-sensitive genotype, was grown in aerated nutrient solution, either with non-saline (control) or 30mM NaCl treatment. At flowering, stems were either infused with sucrose solution (0.44M), water only or maintained without any infusion, for 75 d. The sucrose and water infusion treatments of non-saline plants had no effect on growth or yield, but photosynthesis declined in response to sucrose infusion. Salt stress reduced photosynthesis, decreased tissue sugars by 22-47%, and vegetative and reproductive growth were severely impaired. Sucrose infusion of salt-treated plants increased total sugars in stems, leaves and developing pods, to levels similar to those of non-saline plants. In salt-stressed plants, sucrose infusion increased dry mass (2.6-fold), pod numbers (3.8-fold), seed numbers (6.5-fold) and seed yield (10.4-fold), yet vegetative growth and reproductive failure were not rescued completely by sucrose infusion. Sucrose infusion partly rescued reproductive failure in chickpea by increasing vegetative growth enabling more flower production and by providing sucrose for pod and seed growth. We conclude that insuffcient assimilate availability limits yield in salt-stressed chickpea.

AB - Reproductive processes of chickpea (Cicer arietinum L.) are particularly sensitive to salinity. We tested whether limited photoassimilate availability contributes to reproductive failure in salt-stressed chickpea. Rupali, a salt-sensitive genotype, was grown in aerated nutrient solution, either with non-saline (control) or 30mM NaCl treatment. At flowering, stems were either infused with sucrose solution (0.44M), water only or maintained without any infusion, for 75 d. The sucrose and water infusion treatments of non-saline plants had no effect on growth or yield, but photosynthesis declined in response to sucrose infusion. Salt stress reduced photosynthesis, decreased tissue sugars by 22-47%, and vegetative and reproductive growth were severely impaired. Sucrose infusion of salt-treated plants increased total sugars in stems, leaves and developing pods, to levels similar to those of non-saline plants. In salt-stressed plants, sucrose infusion increased dry mass (2.6-fold), pod numbers (3.8-fold), seed numbers (6.5-fold) and seed yield (10.4-fold), yet vegetative growth and reproductive failure were not rescued completely by sucrose infusion. Sucrose infusion partly rescued reproductive failure in chickpea by increasing vegetative growth enabling more flower production and by providing sucrose for pod and seed growth. We conclude that insuffcient assimilate availability limits yield in salt-stressed chickpea.

KW - Chickpea (Cicer arietinum L.)

KW - Flowering

KW - Photosynthate supply and demand

KW - Photosynthesis

KW - Plant sucrose infusion

KW - Podding

KW - Salinity stress

KW - Seed growth

KW - Tissue ions

KW - Tissue sugars

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U2 - 10.1093/jxb/erw177

DO - 10.1093/jxb/erw177

M3 - Article

VL - 68

SP - 2001

EP - 2011

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

IS - 8

ER -