Vectorial birefringence imaging by optical coherence microscopy for assessing fibrillar microstructures in the cornea and limbus

Qingyun Li, Karol Karnowski, Gavrielle Untracht, Peter B. Noble, Barry Cense, Martin Villiger, David D. Sampson

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The organization of fibrillar tissue on the micrometer scale carries direct implications for health and disease but remains difficult to assess in vivo. Polarization-sensitive optical coherence tomography measures birefringence, which relates to the microscopic arrangement of fibrillar tissue components. Here, we demonstrate a critical improvement in leveraging this contrast mechanism by employing the improved spatial resolution of focus-extended optical coherence microscopy (1.4 µm axially in air and 1.6 µm laterally, over more than 70 µm depth of field). Vectorial birefringence imaging of sheep cornea ex vivo reveals its lamellar organization into thin sections with distinct local optic axis orientations, paving the way to resolving similar features in vivo.

Original languageEnglish
Pages (from-to)1122-1138
Number of pages17
JournalBiomedical Optics Express
Volume11
Issue number2
DOIs
Publication statusPublished - 1 Feb 2020

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