Validity and reliability evidence of a point of care assessment of salivary cortisol and α-amylase: A pre-registered study

Kagan J. Ducker, Robin L.J. Lines, Michael T. Chapman, Peter Peeling, Alannah K.A. McKay, Daniel F. Gucciardi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Purpose. The iPro Cube is a small portable point-of-care device designed to analyse salivary markers of stress in a user-friendly manner (e.g., fast, convenient). Our aim was to test the reliability and validity of the iPro Cube to measure salivary cortisol and α-amylase as compared to the common laboratory standard method (ELISA immunoassay) prior to and after moderate intensity exercise. Methods. The study was a repeated measures, pre-registered design, and statistical framework that incorporated prior knowledge directly into the estimation process. Twenty-nine individuals (age = 27.4 ± 6.6 y; body-mass = 70.8 ± 11.3 kg; height = 1.74 ± 0.92 m; 18 males) completed a single PWC75%HRmax, with repeated measures of salivary cortisol and É.-amylase pre, immediately post, and 30 min post-exercise. Results. Correlation between the iPro Cube and laboratory-based assessments of salivary cortisol was moderate-to-large (0.53 > r < 0.81) across all three testing points. In contrast, correlation between the iPro Cube and laboratory-based assessments of É.-amylase was small-to-moderate (0.25 > r < 0.46). We found a large correlation between duplicate samples of iPro Cube cortisol assessment (0.75 > r < 0.82), and a moderate-to-large correlation for É.-amylase (0.51> r < 0.77). Conclusions. The iPro Cube is capable of taking measures of salivary cortisol that are moderately correlated to values obtained via ELISA immunoassay, however the unit underestimates salivary cortisol and overestimates salivary É.-amylase at rest and post-moderate intensity exercise. It is recommended that researchers continue using standard laboratory techniques to assess these salivary stress markers.

Original languageEnglish
Article number8366
Number of pages15
JournalPEERJ
Volume8
DOIs
Publication statusPublished - 8 Jan 2020

Fingerprint

Dive into the research topics of 'Validity and reliability evidence of a point of care assessment of salivary cortisol and α-amylase: A pre-registered study'. Together they form a unique fingerprint.

Cite this