Validation of low activity 14C-urea breath test in diagnosis of Helicobacter pylori in humans

W. Bielański; S. J. Konturek; M. J. Dobrzańska; M. Płonka; E. Sito; J. Stachura; S. R. Hoffman; B. J. Marshall

Validation of low activity ¹⁴C-urea breath test in diagnosis of Helicobacter pylori in humans

W. Bielański, S. J. Konturek, M. J. Dobrzańska, M. Płonka, E. Sito, J. Stachura, S. R. Hoffman, B. J. Marshall

Research output: Contribution to journal › Article › peer-review

Abstract

Etiologic role for Helicobacter pylori (HP) seems to be well established in gastric pathology. The high urease activity of HP can be used to detect this bacterium by non-invasive urea breath tests (UBT). We validated the low activity version of the test in which 37 kBq ¹⁴C-urea is given orally in capsule. With the cut off value ≥ 1.66 Bq (100 DPM) as positive, UBT results correlated highly significant with combined results for invasive methods i.e. CLO - test + Histology score. The reproducibility of the test was 100%. The results obtained for the breath test performed locally were almost identical with that read at remote laboratory. The data found for fasting and fed states of subjects agreed in 87%. When ¹⁴C-urea was confined in the mouth of both HP positive and HP negative patients UBT showed the presence of urease activity in the mouth cavity.

Original language	English
Pages (from-to)	57-66
Number of pages	10
Journal	Nukleonika
Volume	41
Issue number	2
Publication status	Published - 1996
Externally published	Yes

Cite this

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title = "Validation of low activity 14C-urea breath test in diagnosis of Helicobacter pylori in humans",

abstract = "Etiologic role for Helicobacter pylori (HP) seems to be well established in gastric pathology. The high urease activity of HP can be used to detect this bacterium by non-invasive urea breath tests (UBT). We validated the low activity version of the test in which 37 kBq 14C-urea is given orally in capsule. With the cut off value ≥ 1.66 Bq (100 DPM) as positive, UBT results correlated highly significant with combined results for invasive methods i.e. CLO - test + Histology score. The reproducibility of the test was 100%. The results obtained for the breath test performed locally were almost identical with that read at remote laboratory. The data found for fasting and fed states of subjects agreed in 87%. When 14C-urea was confined in the mouth of both HP positive and HP negative patients UBT showed the presence of urease activity in the mouth cavity.",

author = "W. Biela{\'n}ski and Konturek, {S. J.} and Dobrza{\'n}ska, {M. J.} and M. P{\l}onka and E. Sito and J. Stachura and Hoffman, {S. R.} and Marshall, {B. J.}",

year = "1996",

language = "English",

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journal = "Nukleonika",

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TY - JOUR

T1 - Validation of low activity 14C-urea breath test in diagnosis of Helicobacter pylori in humans

AU - Bielański, W.

AU - Konturek, S. J.

AU - Dobrzańska, M. J.

AU - Płonka, M.

AU - Sito, E.

AU - Stachura, J.

AU - Hoffman, S. R.

AU - Marshall, B. J.

PY - 1996

Y1 - 1996

N2 - Etiologic role for Helicobacter pylori (HP) seems to be well established in gastric pathology. The high urease activity of HP can be used to detect this bacterium by non-invasive urea breath tests (UBT). We validated the low activity version of the test in which 37 kBq 14C-urea is given orally in capsule. With the cut off value ≥ 1.66 Bq (100 DPM) as positive, UBT results correlated highly significant with combined results for invasive methods i.e. CLO - test + Histology score. The reproducibility of the test was 100%. The results obtained for the breath test performed locally were almost identical with that read at remote laboratory. The data found for fasting and fed states of subjects agreed in 87%. When 14C-urea was confined in the mouth of both HP positive and HP negative patients UBT showed the presence of urease activity in the mouth cavity.

AB - Etiologic role for Helicobacter pylori (HP) seems to be well established in gastric pathology. The high urease activity of HP can be used to detect this bacterium by non-invasive urea breath tests (UBT). We validated the low activity version of the test in which 37 kBq 14C-urea is given orally in capsule. With the cut off value ≥ 1.66 Bq (100 DPM) as positive, UBT results correlated highly significant with combined results for invasive methods i.e. CLO - test + Histology score. The reproducibility of the test was 100%. The results obtained for the breath test performed locally were almost identical with that read at remote laboratory. The data found for fasting and fed states of subjects agreed in 87%. When 14C-urea was confined in the mouth of both HP positive and HP negative patients UBT showed the presence of urease activity in the mouth cavity.

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M3 - Article

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Validation of low activity ¹⁴C-urea breath test in diagnosis of Helicobacter pylori in humans

Abstract

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