Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections

Mispah Mukap, Corin Sprod, Nakapi Teguarani, Moses Laman, Madhu Page-Sharp, Sam Salman, Brioni Moore, Kevin Batty, Timothy Davis, Laurens Manning

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95% confidence interval 0.91-0.97], P
Original languageEnglish
Article numbere00940
JournalAntimicrobial Agents and Chemotherapy
Volume62
Issue number10
Early online date2018
DOIs
Publication statusPublished - Oct 2018

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Ceftriaxone
Bacterial Infections
Papua New Guinea
Pharmacokinetics
Bacterial Meningitides
Hematocrit
Liquid Chromatography
Anemia
Mass Spectrometry
Pneumonia
Confidence Intervals
Anti-Bacterial Agents
Delivery of Health Care
Population

Cite this

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title = "Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections",
abstract = "Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95{\%} confidence interval 0.91-0.97], P",
author = "Mispah Mukap and Corin Sprod and Nakapi Teguarani and Moses Laman and Madhu Page-Sharp and Sam Salman and Brioni Moore and Kevin Batty and Timothy Davis and Laurens Manning",
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language = "English",
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journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
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Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections. / Mukap, Mispah; Sprod, Corin; Teguarani, Nakapi; Laman, Moses; Page-Sharp, Madhu; Salman, Sam; Moore, Brioni; Batty, Kevin; Davis, Timothy; Manning, Laurens.

In: Antimicrobial Agents and Chemotherapy, Vol. 62, No. 10, e00940, 10.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections

AU - Mukap, Mispah

AU - Sprod, Corin

AU - Teguarani, Nakapi

AU - Laman, Moses

AU - Page-Sharp, Madhu

AU - Salman, Sam

AU - Moore, Brioni

AU - Batty, Kevin

AU - Davis, Timothy

AU - Manning, Laurens

PY - 2018/10

Y1 - 2018/10

N2 - Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95% confidence interval 0.91-0.97], P

AB - Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95% confidence interval 0.91-0.97], P

U2 - 10.1128/AAC.00940-18

DO - 10.1128/AAC.00940-18

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JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

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