Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections

Mispah Mukap, Corin Sprod, Nakapi Teguarani, Moses Laman, Madhu Page-Sharp, Sam Salman, Brioni Moore, Kevin Batty, Timothy Davis, Laurens Manning

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95% confidence interval 0.91-0.97], P
Original languageEnglish
Article numbere00940
JournalAntimicrobial Agents and Chemotherapy
Volume62
Issue number10
Early online date2018
DOIs
Publication statusPublished - Oct 2018

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