Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections

Mispah Mukap; Corin Sprod; Nakapi Teguarani; Moses Laman; Madhu Page-Sharp; Sam Salman; Brioni Moore; Kevin Batty; Timothy Davis; Laurens Manning

doi:10.1128/AAC.00940-18

Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections

Mispah Mukap, Corin Sprod, Nakapi Teguarani, Moses Laman, Madhu Page-Sharp, Sam Salman, Brioni Moore, Kevin Batty, Timothy Davis, Laurens Manning

UWA Medical School

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95% confidence interval 0.91-0.97], P

Original language	English
Article number	e00940
Journal	Antimicrobial Agents and Chemotherapy
Volume	62
Issue number	10
Early online date	2018
DOIs	https://doi.org/10.1128/AAC.00940-18
Publication status	Published - Oct 2018

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10.1128/AAC.00940-18

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6153847/

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title = "Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections",

abstract = "Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95% confidence interval 0.91-0.97], P",

author = "Mispah Mukap and Corin Sprod and Nakapi Teguarani and Moses Laman and Madhu Page-Sharp and Sam Salman and Brioni Moore and Kevin Batty and Timothy Davis and Laurens Manning",

year = "2018",

month = oct,

doi = "10.1128/AAC.00940-18",

language = "English",

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journal = "Antimicrobial Agents and Chemotherapy",

issn = "1098-6596",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections

AU - Mukap, Mispah

AU - Sprod, Corin

AU - Teguarani, Nakapi

AU - Laman, Moses

AU - Page-Sharp, Madhu

AU - Salman, Sam

AU - Moore, Brioni

AU - Batty, Kevin

AU - Davis, Timothy

AU - Manning, Laurens

PY - 2018/10

Y1 - 2018/10

N2 - Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95% confidence interval 0.91-0.97], P

AB - Background: Dried blood spot (DBS) antibiotic assays can facilitate pharmacokinetic (PK) studies in situations where venous blood sampling is logistically and/or ethically challenging. In this study we aimed to demonstrate the validity of a DBS ceftriaxone assay in a PK study of children from Papua New Guinea (PNG) with severe illness, a setting in which healthcare resources are limited and anemia is common. Methods: Using a previously validated liquid chromatography-mass spectroscopy (LC-MS/MS) assay, serial plasma and DBS ceftriaxone concentrations were measured in PNG children aged 5-10 years with acute bacterial meningitis or severe pneumonia. The concentration-time data were incorporated into population PK models. Results: Ten children were recruited with an admission hematocrit of 0.22-0.52. Raw data demonstrated good correlation between plasma and DBS concentrations (Spearman rs =0.94 [95% confidence interval 0.91-0.97], P

U2 - 10.1128/AAC.00940-18

DO - 10.1128/AAC.00940-18

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SN - 1098-6596

VL - 62

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 10

M1 - e00940

ER -

Validation of a dried blood spot ceftriaxone assay in Papua New Guinean children with severe bacterial infections

Abstract

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