Uveal mast cells are not required for rodent uveitis

Justine R. Smith, Prue H. Hart, Douglas J. Coster, Keryn A. Williams

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Uveal mast cells have previously been considered to be vital mediators of experimental uveitis. We extended the study of these cells to experimental melanin-induced uveitis (EMIU), a recently described clinically relevant model, and re-examined their role in endotoxin-induced uveitis (EIU) using genetically mast cell-depleted mice on a single background. EMIU was induced in Fischer 344 rats by immunisation with bovine ocular melanin (250 μg). Animals were killed immediately, and on days 1 and 3 of clinical disease. Numbers of uveal mast cells and the percentage of degranulated cells were counted in whole-mount preparations. There was no significant change in either measure across the selected time points. To induce EIU, normal and mast cell-depleted DBA/2 mice were injected with Escherichia coli lipopolysaccharide (400 μg). Cells infiltrating the eye 24 h after injection were quantified in 5 μm ocular cross-sections. Disease was not significantly reduced in the mast cell-depleted mutants. We conclude that uveal mast cells are not required for the development of EMIU or, in contrast to earlier work, EIU.

Original languageEnglish
Pages (from-to)388-393
Number of pages6
JournalOphthalmic research
Volume30
Issue number6
DOIs
Publication statusPublished - 1 Nov 1998
Externally publishedYes

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