Using ’omics technologies to understand pathogenesis and seek alternative therapies for otitis media in children

Rachael Lappan

doi:10.26182/5cf73acaee262

Using ’omics technologies to understand pathogenesis and seek alternative therapies for otitis media in children

Rachael Lappan

School of Biomedical Sciences

Research output: Thesis › Doctoral Thesis

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Abstract

Recurrent acute otitis media (rAOM, recurrent ear infection) is a common childhood disease, for which treatment is not always effective. This thesis describes the use of next-generation sequencing approaches to 1) investigate novel pathogens in rAOM; and 2) identify protective microbes for translation into therapy. Alloiococcus otitidis and Turicella otitidis were dominant organisms in rAOM, but are questionably pathogenic. Corynebacterium pseudodiphtheriticum inhibited the growth of an rAOM pathogen. This thesis also details the methodological challenges in applying metagenomics and metatranscriptomics to rAOM; knowledge which is essential for further 'omics-based studies in the upper respiratory tract.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Peacock, Christopher, Supervisor Jamieson, Sarra, Supervisor Beilharz, Manfred, Supervisor Lassmann, Timo, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	7 Apr 2019
DOIs	https://doi.org/10.26182/5cf73acaee262
Publication status	Unpublished - 2019

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10.26182/5cf73acaee262

THESIS - DOCTOR OF PHILOSOPHY - LAPPAN, Rachael Joanna - 2019
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Cite this

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title = "Using {\textquoteright}omics technologies to understand pathogenesis and seek alternative therapies for otitis media in children",

abstract = "Recurrent acute otitis media (rAOM, recurrent ear infection) is a common childhood disease, for which treatment is not always effective. This thesis describes the use of next-generation sequencing approaches to 1) investigate novel pathogens in rAOM; and 2) identify protective microbes for translation into therapy. Alloiococcus otitidis and Turicella otitidis were dominant organisms in rAOM, but are questionably pathogenic. Corynebacterium pseudodiphtheriticum inhibited the growth of an rAOM pathogen. This thesis also details the methodological challenges in applying metagenomics and metatranscriptomics to rAOM; knowledge which is essential for further 'omics-based studies in the upper respiratory tract.",

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AB - Recurrent acute otitis media (rAOM, recurrent ear infection) is a common childhood disease, for which treatment is not always effective. This thesis describes the use of next-generation sequencing approaches to 1) investigate novel pathogens in rAOM; and 2) identify protective microbes for translation into therapy. Alloiococcus otitidis and Turicella otitidis were dominant organisms in rAOM, but are questionably pathogenic. Corynebacterium pseudodiphtheriticum inhibited the growth of an rAOM pathogen. This thesis also details the methodological challenges in applying metagenomics and metatranscriptomics to rAOM; knowledge which is essential for further 'omics-based studies in the upper respiratory tract.

KW - Otitis media

KW - Microbiome

KW - Child health

KW - Infection

U2 - 10.26182/5cf73acaee262

DO - 10.26182/5cf73acaee262

M3 - Doctoral Thesis

ER -

Using ’omics technologies to understand pathogenesis and seek alternative therapies for otitis media in children

Abstract

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