Use of RAPD-PCR to isolate a species specific DNA probe for Phytophthora cinnamomi

Mark P. Dobrowolski, Philip A. O'Brien

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The products of RAPD-PCR amplification of Phytophthora cinnamomi DNA were separated by electrophoresis in agarose. Parallel Southern blots of the gels were hybridized with nick translated DNA from different species of Phytophthora. Fragments that hybridized specifically to P. cinnamomi DNA were identified. These fragments were purified and cloned into pUC18. Their specificity for P. cinnamomi was confirmed.

Original languageEnglish
Pages (from-to)43-47
Number of pages5
JournalFEMS Microbiology Letters
Volume113
Issue number1
Publication statusPublished - 1 Oct 1993
Externally publishedYes

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