Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells

Karen A F Pulford, Wendy N. Erber, Julie A. Crick, Inge Olsson, K. J. Micklem, K. C. Gatter, D. Y. Mason

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68% of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern-that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.

Original languageEnglish
Pages (from-to)853-860
Number of pages8
JournalJournal of Clinical Pathology
Volume41
Issue number8
DOIs
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Leukocyte Elastase
Pancreatic Elastase
Myeloid Cells
Neutrophils
Monoclonal Antibodies
Myeloid Leukemia
Peroxidase
Paraffin
Anti-Idiotypic Antibodies
Coloring Agents
Leukemia, Monocytic, Acute
Lymphoid Leukemia
Cell Line
Waxes
Phosphoric Monoester Hydrolases
Acute Myeloid Leukemia
Alkaline Phosphatase
Monocytes
Leukemia
Differential Diagnosis

Cite this

Pulford, K. A. F., Erber, W. N., Crick, J. A., Olsson, I., Micklem, K. J., Gatter, K. C., & Mason, D. Y. (1988). Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells. Journal of Clinical Pathology, 41(8), 853-860. https://doi.org/10.1136/jcp.41.8.853
Pulford, Karen A F ; Erber, Wendy N. ; Crick, Julie A. ; Olsson, Inge ; Micklem, K. J. ; Gatter, K. C. ; Mason, D. Y. / Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells. In: Journal of Clinical Pathology. 1988 ; Vol. 41, No. 8. pp. 853-860.
@article{caa00e9724514c27a4135b867cd70d1e,
title = "Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells",
abstract = "A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68{\%} of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern-that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.",
author = "Pulford, {Karen A F} and Erber, {Wendy N.} and Crick, {Julie A.} and Inge Olsson and Micklem, {K. J.} and Gatter, {K. C.} and Mason, {D. Y.}",
year = "1988",
doi = "10.1136/jcp.41.8.853",
language = "English",
volume = "41",
pages = "853--860",
journal = "Journal of Clinical Pathology",
issn = "0021-9746",
publisher = "BMJ Publishing Group",
number = "8",

}

Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells. / Pulford, Karen A F; Erber, Wendy N.; Crick, Julie A.; Olsson, Inge; Micklem, K. J.; Gatter, K. C.; Mason, D. Y.

In: Journal of Clinical Pathology, Vol. 41, No. 8, 1988, p. 853-860.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells

AU - Pulford, Karen A F

AU - Erber, Wendy N.

AU - Crick, Julie A.

AU - Olsson, Inge

AU - Micklem, K. J.

AU - Gatter, K. C.

AU - Mason, D. Y.

PY - 1988

Y1 - 1988

N2 - A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68% of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern-that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.

AB - A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68% of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern-that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.

UR - http://www.scopus.com/inward/record.url?scp=0023759436&partnerID=8YFLogxK

U2 - 10.1136/jcp.41.8.853

DO - 10.1136/jcp.41.8.853

M3 - Article

VL - 41

SP - 853

EP - 860

JO - Journal of Clinical Pathology

JF - Journal of Clinical Pathology

SN - 0021-9746

IS - 8

ER -