Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells

Karen A F Pulford, Wendy N. Erber, Julie A. Crick, Inge Olsson, K. J. Micklem, K. C. Gatter, D. Y. Mason

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

A monoclonal antibody, NP57, was produced and used against the neutrophil granule protein elastase, which selectively stain neutrophils in cryostat and paraffin wax sections. The antibody stains neutrophils and a subpopulation of monocytes in blood smears and neutrophil precursors in bone marrow smears, and gives positive reactions with the cell lines HL60 and U-937. It labelled the blast cells in 68% of cases of acute myeloid leukaemia (M1-M5) but was unreactive with all cases of lymphoid leukaemias. Most of the elastase negative myeloid leukaemias were labelled by monoclonal anti-myeloperoxidase (antibody MPO-7) as were cells from the promyelocytic line HL60. No cases of myeloid leukaemia showed the opposite pattern-that is elastase positive, myeloperoxidase negative, suggesting that the production of myeloperoxidase precedes the onset of elastase synthesis during myeloid maturation. The anti-elastase antibody NP57 is a useful addition to the range of monoclonal antibodies available for the differential diagnosis of acute leukaemia by alkaline phosphatase-antialkaline phosphatase (APAAP) labelling of cell smears; it may also be of value for the histopathological diagnosis of tumour deposits in myeloid leukaemia and for the detection of neutrophils in paraffin sections.

Original languageEnglish
Pages (from-to)853-860
Number of pages8
JournalJournal of Clinical Pathology
Volume41
Issue number8
DOIs
Publication statusPublished - 1988
Externally publishedYes

Fingerprint Dive into the research topics of 'Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells'. Together they form a unique fingerprint.

  • Cite this

    Pulford, K. A. F., Erber, W. N., Crick, J. A., Olsson, I., Micklem, K. J., Gatter, K. C., & Mason, D. Y. (1988). Use of monoclonal antibody against human neutrophil elastase in normal and leukaemic myeloid cells. Journal of Clinical Pathology, 41(8), 853-860. https://doi.org/10.1136/jcp.41.8.853