Uptake of FITC-Chitosan Nanoparticles by A549 Cells

M. Huang, Z. Ma, E. Khor, Lee Yong Lim

    Research output: Contribution to journalArticlepeer-review

    321 Citations (Scopus)

    Abstract

    Purpose. The objective of this study was to evaluate the extent and mechanism of uptake of fluorescent chitosan nanoparticles by the A549 cells, a human cell line derived from the respiratory epithelium.Methods. Covalent conjugation with fluorescein-5-isothiocyanate yielded stably labeled chitosan molecules, which were successfully formulated into nanoparticles by ionotropic gelation. Uptake of fluorescein-5-isothiocyanate-chitosan nanoparticles and chitosan molecules by confluent A549 cells was quantified by fluorometry.Results. Cellular uptake of chitosan nanoparticles was concentration and temperature dependent, having K-m and V-max of 3.84 muM and 58.14 mug/mg protein/h, respectively. Uptake of chitosan nanoparticles was up to 1.8-fold higher than that of chitosan molecules alone and was not inhibited by excess unlabeled chitosan molecules. Hyperosmolarity, chlorpromazine and K+ depletion inhibited by 65, 34, and 54%, respectively, the uptake of chitosan nanoparticles at 37degreesC, but filipin had no influence on the uptake. Confocal imaging confirmed the internalization of the chitosan nanoparticles by the A549 cells at 37degreesC.Conclusions. Formulation of chitosan into nanoparticles significantly improved its uptake by the A549 cells. Internalization of chitosan nanoparticles by the cells seems to occur predominantly by adsorptive endocytosis initiated by nonspecific interactions between nanoparticles and cell membranes, and was in part mediated by clathrin-mediated process.
    Original languageEnglish
    Pages (from-to)1488-1494
    JournalPharmaceutical Research
    Volume19
    Issue number10
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Dive into the research topics of 'Uptake of FITC-Chitosan Nanoparticles by A549 Cells'. Together they form a unique fingerprint.

    Cite this