Some Proteaceae of south-western Australia show delayed-greening leaf development. This was investigated in Hakea prostrata R. Br. in relation to early leaf development. I show the molecular underpinnings of metabolite and protein profiles during leaf development in delayed-greening species. The temporal separation of leaf structural development and chloroplast development was reflected in the distribution of metabolite and protein profiles during leaf development. This is the first study to research delayed greening phenomenon through novel techniques of metabolomics and proteomics and will provide the basis for future specific and targeted analysis of delayed greening at the molecular level.