Under-nutrition reduces spermatogenic efficiency and sperm velocity, and increases sperm DNA damage in sexually mature male sheep

Yongjuan Guan, Irek Malecki, Penny Hawken, Matthew Linden, Graeme Martin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

© 2014 Elsevier B.V. We tested whether the quality of spermatozoa from mature male sheep would be affected during nutrition-induced changes in testicular mass. Merino rams were fed for 65 days with diets that increased, maintained or decreased body and testis mass (n = 8 per group). In semen collected on Days 56 and 63, underfed rams had less sperms per ejaculate than well-fed rams (P <0.05) and a lower sperm velocity (computer-assisted semen analysis) than well-fed or maintenance-fed rams (P <0.05). Sperm chromatin structure assay revealed more sperm DNA damage in underfed rams than in well-fed rams (P <0.05). The amount of sperm DNA damage was inversely correlated with change in scrotal circumference (r = -0.6, P <0.05), the percentages of progressive motile sperm (r = -0.8; P <0.01) and motile sperm (r = -0.6, P <0.05), and the numbers of sperms per gram of testis (r = -0.55, P <0.05). In testicular tissue collected on Day 65, underfed rams had fewer sperm per gram of testis than rams in the other two groups (P <0.001). We conclude that, in adult rams, underfeeding reduces spermatogenic efficiency and that this response is associated with a reduction in spermatozoal quality.
Original languageEnglish
Pages (from-to)163-172
JournalAnimal Reproduction Science
Volume149
Issue number3-4
DOIs
Publication statusPublished - 2014

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DNA damage
malnutrition
DNA Damage
Spermatozoa
rams
Sheep
spermatozoa
sheep
Testis
testes
semen
Semen Analysis
Sperm Count
Semen
restricted feeding
Merino
Chromatin
chromatin
Maintenance
Diet

Cite this

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title = "Under-nutrition reduces spermatogenic efficiency and sperm velocity, and increases sperm DNA damage in sexually mature male sheep",
abstract = "{\circledC} 2014 Elsevier B.V. We tested whether the quality of spermatozoa from mature male sheep would be affected during nutrition-induced changes in testicular mass. Merino rams were fed for 65 days with diets that increased, maintained or decreased body and testis mass (n = 8 per group). In semen collected on Days 56 and 63, underfed rams had less sperms per ejaculate than well-fed rams (P <0.05) and a lower sperm velocity (computer-assisted semen analysis) than well-fed or maintenance-fed rams (P <0.05). Sperm chromatin structure assay revealed more sperm DNA damage in underfed rams than in well-fed rams (P <0.05). The amount of sperm DNA damage was inversely correlated with change in scrotal circumference (r = -0.6, P <0.05), the percentages of progressive motile sperm (r = -0.8; P <0.01) and motile sperm (r = -0.6, P <0.05), and the numbers of sperms per gram of testis (r = -0.55, P <0.05). In testicular tissue collected on Day 65, underfed rams had fewer sperm per gram of testis than rams in the other two groups (P <0.001). We conclude that, in adult rams, underfeeding reduces spermatogenic efficiency and that this response is associated with a reduction in spermatozoal quality.",
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Under-nutrition reduces spermatogenic efficiency and sperm velocity, and increases sperm DNA damage in sexually mature male sheep. / Guan, Yongjuan; Malecki, Irek; Hawken, Penny; Linden, Matthew; Martin, Graeme.

In: Animal Reproduction Science, Vol. 149, No. 3-4, 2014, p. 163-172.

Research output: Contribution to journalArticle

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AU - Malecki, Irek

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AU - Linden, Matthew

AU - Martin, Graeme

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N2 - © 2014 Elsevier B.V. We tested whether the quality of spermatozoa from mature male sheep would be affected during nutrition-induced changes in testicular mass. Merino rams were fed for 65 days with diets that increased, maintained or decreased body and testis mass (n = 8 per group). In semen collected on Days 56 and 63, underfed rams had less sperms per ejaculate than well-fed rams (P <0.05) and a lower sperm velocity (computer-assisted semen analysis) than well-fed or maintenance-fed rams (P <0.05). Sperm chromatin structure assay revealed more sperm DNA damage in underfed rams than in well-fed rams (P <0.05). The amount of sperm DNA damage was inversely correlated with change in scrotal circumference (r = -0.6, P <0.05), the percentages of progressive motile sperm (r = -0.8; P <0.01) and motile sperm (r = -0.6, P <0.05), and the numbers of sperms per gram of testis (r = -0.55, P <0.05). In testicular tissue collected on Day 65, underfed rams had fewer sperm per gram of testis than rams in the other two groups (P <0.001). We conclude that, in adult rams, underfeeding reduces spermatogenic efficiency and that this response is associated with a reduction in spermatozoal quality.

AB - © 2014 Elsevier B.V. We tested whether the quality of spermatozoa from mature male sheep would be affected during nutrition-induced changes in testicular mass. Merino rams were fed for 65 days with diets that increased, maintained or decreased body and testis mass (n = 8 per group). In semen collected on Days 56 and 63, underfed rams had less sperms per ejaculate than well-fed rams (P <0.05) and a lower sperm velocity (computer-assisted semen analysis) than well-fed or maintenance-fed rams (P <0.05). Sperm chromatin structure assay revealed more sperm DNA damage in underfed rams than in well-fed rams (P <0.05). The amount of sperm DNA damage was inversely correlated with change in scrotal circumference (r = -0.6, P <0.05), the percentages of progressive motile sperm (r = -0.8; P <0.01) and motile sperm (r = -0.6, P <0.05), and the numbers of sperms per gram of testis (r = -0.55, P <0.05). In testicular tissue collected on Day 65, underfed rams had fewer sperm per gram of testis than rams in the other two groups (P <0.001). We conclude that, in adult rams, underfeeding reduces spermatogenic efficiency and that this response is associated with a reduction in spermatozoal quality.

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