Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field

Andrea Curatolo, M. Villiger, Dirk Lorenser, Philip Wijesinghe, Alexander Fritz, Brendan F. Kennedy, David D. Sampson

Research output: Chapter in Book/Conference paperConference paper

Abstract

Copyright © 2016 SPIE.Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 µm transverse resolution over nearly 100 µm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 µm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.
Original languageEnglish
Title of host publicationProceedings of SPIE: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
EditorsJ. A. Izatt , J. G. Fujimoto, V. V. Tuchin
PublisherS P I E - International Society for Optical Engineering
Volume9697
ISBN (Print)9781628419313
DOIs
Publication statusPublished - 2016
EventOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX - San Francisco, United States
Duration: 15 Feb 201617 Feb 2016

Conference

ConferenceOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
CountryUnited States
CitySan Francisco
Period15/02/1617/02/16

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