Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field

Andrea Curatolo, M. Villiger, Dirk Lorenser, Philip Wijesinghe, Alexander Fritz, Brendan F. Kennedy, David D. Sampson

Research output: Chapter in Book/Conference paperConference paper

Abstract

Copyright © 2016 SPIE.Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 µm transverse resolution over nearly 100 µm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 µm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.
Original languageEnglish
Title of host publicationProceedings of SPIE: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
EditorsJ. A. Izatt , J. G. Fujimoto, V. V. Tuchin
PublisherS P I E - International Society for Optical Engineering
Volume9697
ISBN (Print)9781628419313
DOIs
Publication statusPublished - 2016
EventOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX - San Francisco, United States
Duration: 15 Feb 201617 Feb 2016

Conference

ConferenceOptical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX
CountryUnited States
CitySan Francisco
Period15/02/1617/02/16

Fingerprint

elastin
smooth muscle
muscle cells
aorta
axial strain
progressions
mice
stiffness
illumination
microscopes
inclusions
bandwidth
sensitivity

Cite this

Curatolo, A., Villiger, M., Lorenser, D., Wijesinghe, P., Fritz, A., Kennedy, B. F., & Sampson, D. D. (2016). Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field. In J. A. Izatt , J. G. Fujimoto, & V. V. Tuchin (Eds.), Proceedings of SPIE: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX (Vol. 9697). [96971Q] S P I E - International Society for Optical Engineering. https://doi.org/10.1117/12.2214684
Curatolo, Andrea ; Villiger, M. ; Lorenser, Dirk ; Wijesinghe, Philip ; Fritz, Alexander ; Kennedy, Brendan F. ; Sampson, David D. / Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field. Proceedings of SPIE: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX. editor / J. A. Izatt ; J. G. Fujimoto ; V. V. Tuchin. Vol. 9697 S P I E - International Society for Optical Engineering, 2016.
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Curatolo, A, Villiger, M, Lorenser, D, Wijesinghe, P, Fritz, A, Kennedy, BF & Sampson, DD 2016, Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field. in JA Izatt , JG Fujimoto & VV Tuchin (eds), Proceedings of SPIE: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX. vol. 9697, 96971Q, S P I E - International Society for Optical Engineering, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX, San Francisco, United States, 15/02/16. https://doi.org/10.1117/12.2214684

Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field. / Curatolo, Andrea; Villiger, M.; Lorenser, Dirk; Wijesinghe, Philip; Fritz, Alexander; Kennedy, Brendan F.; Sampson, David D.

Proceedings of SPIE: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX. ed. / J. A. Izatt ; J. G. Fujimoto; V. V. Tuchin. Vol. 9697 S P I E - International Society for Optical Engineering, 2016. 96971Q.

Research output: Chapter in Book/Conference paperConference paper

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T1 - Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field

AU - Curatolo, Andrea

AU - Villiger, M.

AU - Lorenser, Dirk

AU - Wijesinghe, Philip

AU - Fritz, Alexander

AU - Kennedy, Brendan F.

AU - Sampson, David D.

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N2 - Copyright © 2016 SPIE.Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 µm transverse resolution over nearly 100 µm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 µm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.

AB - Copyright © 2016 SPIE.Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 µm transverse resolution over nearly 100 µm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 µm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.

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Curatolo A, Villiger M, Lorenser D, Wijesinghe P, Fritz A, Kennedy BF et al. Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field. In Izatt JA, Fujimoto JG, Tuchin VV, editors, Proceedings of SPIE: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX. Vol. 9697. S P I E - International Society for Optical Engineering. 2016. 96971Q https://doi.org/10.1117/12.2214684