@inproceedings{1760d84293fd41a484284e1f1b52b3ea,
title = "Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field",
abstract = "Copyright {\textcopyright} 2016 SPIE.Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 µm transverse resolution over nearly 100 µm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 µm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.",
author = "Andrea Curatolo and M. Villiger and Dirk Lorenser and Philip Wijesinghe and Alexander Fritz and Kennedy, {Brendan F.} and Sampson, {David D.}",
year = "2016",
doi = "10.1117/12.2214684",
language = "English",
isbn = "9781628419313",
volume = "9697",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
publisher = "S P I E - International Society for Optical Engineering",
editor = "Tuchin, {Valery V.} and Izatt, {Joseph A.} and Fujimoto, {James G.}",
booktitle = "Proceedings of SPIE: Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX",
note = "Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XX ; Conference date: 15-02-2016 Through 17-02-2016",
}