Abstract
In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens microendoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1:6 μm and an axial resolution of 2:2 μm. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 μm, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo.
| Original language | English |
|---|---|
| Article number | 304351 |
| Pages (from-to) | 5127-5138 |
| Number of pages | 12 |
| Journal | Biomedical Optics Express |
| Volume | 8 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 1 Nov 2017 |
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Ultrahigh-resolution optical coherence elastography through a micro-endoscope : Towards in vivo imaging of cellular-scale mechanics. / Fang, Qi; Curatolo, Andrea; Wijesinghe, Philip; Yeow, Yen Ling; Hamzah, Juliana; Noble, Peter B.; Karnowski, Karol; Sampson, David D.; Ganss, Ruth; Kim, Jun Ki; Lee, Woei M.; Kennedy, Brendan F.
In: Biomedical Optics Express, Vol. 8, No. 11, 304351, 01.11.2017, p. 5127-5138.Research output: Contribution to journal › Article
TY - JOUR
T1 - Ultrahigh-resolution optical coherence elastography through a micro-endoscope
T2 - Towards in vivo imaging of cellular-scale mechanics
AU - Fang, Qi
AU - Curatolo, Andrea
AU - Wijesinghe, Philip
AU - Yeow, Yen Ling
AU - Hamzah, Juliana
AU - Noble, Peter B.
AU - Karnowski, Karol
AU - Sampson, David D.
AU - Ganss, Ruth
AU - Kim, Jun Ki
AU - Lee, Woei M.
AU - Kennedy, Brendan F.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens microendoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1:6 μm and an axial resolution of 2:2 μm. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 μm, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo.
AB - In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens microendoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1:6 μm and an axial resolution of 2:2 μm. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 μm, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo.
UR - http://www.scopus.com/inward/record.url?scp=85032811234&partnerID=8YFLogxK
U2 - 10.1364/BOE.8.005127
DO - 10.1364/BOE.8.005127
M3 - Article
VL - 8
SP - 5127
EP - 5138
JO - Biomedical Optics Express
JF - Biomedical Optics Express
SN - 2156-7085
IS - 11
M1 - 304351
ER -