Ultrahigh-resolution optical coherence elastography through a micro-endoscope: Towards in vivo imaging of cellular-scale mechanics

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Abstract

In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens microendoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1:6 μm and an axial resolution of 2:2 μm. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 μm, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo.

Original languageEnglish
Article number304351
Pages (from-to)5127-5138
Number of pages12
JournalBiomedical Optics Express
Volume8
Issue number11
DOIs
Publication statusPublished - 1 Nov 2017

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Elasticity Imaging Techniques
endoscopes
Endoscopes
Mechanics
Biological Phenomena
Optical Devices
Elasticity
Silicones
Lighting
Lenses
Disease Progression
silicones
muscles
progressions
Muscles
tumors
elastic properties
illumination
lenses
mechanical properties

Cite this

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title = "Ultrahigh-resolution optical coherence elastography through a micro-endoscope: Towards in vivo imaging of cellular-scale mechanics",
abstract = "In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens microendoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1:6 μm and an axial resolution of 2:2 μm. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 μm, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo.",
author = "Qi Fang and Andrea Curatolo and Philip Wijesinghe and Yeow, {Yen Ling} and Juliana Hamzah and Noble, {Peter B.} and Karol Karnowski and Sampson, {David D.} and Ruth Ganss and Kim, {Jun Ki} and Lee, {Woei M.} and Kennedy, {Brendan F.}",
year = "2017",
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doi = "10.1364/BOE.8.005127",
language = "English",
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T1 - Ultrahigh-resolution optical coherence elastography through a micro-endoscope

T2 - Towards in vivo imaging of cellular-scale mechanics

AU - Fang, Qi

AU - Curatolo, Andrea

AU - Wijesinghe, Philip

AU - Yeow, Yen Ling

AU - Hamzah, Juliana

AU - Noble, Peter B.

AU - Karnowski, Karol

AU - Sampson, David D.

AU - Ganss, Ruth

AU - Kim, Jun Ki

AU - Lee, Woei M.

AU - Kennedy, Brendan F.

PY - 2017/11/1

Y1 - 2017/11/1

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AB - In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens microendoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1:6 μm and an axial resolution of 2:2 μm. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 μm, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo.

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