Ultrahigh-resolution optical coherence elastography through a micro-endoscope: Towards in vivo imaging of cellular-scale mechanics

Qi Fang, Andrea Curatolo, Philip Wijesinghe, Yen Ling Yeow, Juliana Hamzah, Peter B. Noble, Karol Karnowski, David D. Sampson, Ruth Ganss, Jun Ki Kim, Woei M. Lee, Brendan F. Kennedy

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens microendoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1:6 μm and an axial resolution of 2:2 μm. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 μm, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo.

Original languageEnglish
Article number304351
Pages (from-to)5127-5138
Number of pages12
JournalBiomedical Optics Express
Volume8
Issue number11
DOIs
Publication statusPublished - 1 Nov 2017

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