Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana

Christopher Carrie, Monika Murcha, Kristina Kuehn, Owen Duncan, M. Barthet, P.M. Smith, Holger Eubel, Etienne Meyer, D.A. Day, Harvey Millar, James Whelan

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

We found that four type II NAD(P)H dehydrogenases (ND) in Arabidopsis are targeted to two locations in the cell; NDC1 was targeted to mitochondria and chloroplasts, while NDA1, NDA2 and NDB1 were targeted to mitochondria and peroxisomes. Targeting of NDC1 to chloroplasts as well as mitochondria was shown using in vitro and in vivo uptake assays and dual targeting of NDC1 to plastids relies on regions in the mature part of the protein. Accumulation of NDA type dehydrogenases to peroxisomes and mitochondria was confirmed using Western blot analysis on highly purified organelle fractions. Targeting of ND proteins to mitochondria and peroxisomes is achieved by two separate signals, a C-terminal signal for peroxisomes and an N-terminal signal for mitochondria.
Original languageEnglish
Pages (from-to)3073-3079
JournalFEBS Letters
Volume582
Issue number3
DOIs
Publication statusPublished - 2008

Fingerprint Dive into the research topics of 'Type II NAD(P)H dehydrogenases are targeted to mitochondria and chloroplasts or peroxisomes in Arabidopsis thaliana'. Together they form a unique fingerprint.

Cite this