Tympanic membrane wound healing in rats: a combined histological, immunohistochemical and transcriptome study

Peter Santa Maria

    Research output: ThesisDoctoral Thesis

    508 Downloads (Pure)

    Abstract

    The aim of this research was to elucidate the transcriptional changes that occur in response to tympanic membrane (TM) perforation in rats and to infer key genes and molecular events in the healing process. Research was undertaken to examine the histological changes and the immunohistochemistry of growth factors – specifically keratinocyte growth factor (KGF) and epidermal growth factor (EGF) – in the TM of rats following wound healing. Tissue from 240 individual rats was used in the microarray following random allocation into time point groups to be sacrificed over 7 days. An Agilent one colour microarray technique was performed and the results were analysed using Genespring GX9 software. A total of 3262 genes were identified as significant (p
    Original languageEnglish
    QualificationDoctor of Philosophy
    Publication statusUnpublished - 2010

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