Twin birth changes DNA methylation of subsequent siblings

Shuai Li, Eunae Kim, Ee Ming Wong, Ji Hoon Eric Joo, Tuong L. Nguyen, Jennifer Stone, Yun Mi Song, Louisa B. Flander, Richard Saffery, Graham G. Giles, Melissa C. Southey, Joohon Sung, John L. Hopper

Research output: Contribution to journalArticle

Abstract

We asked if twin birth influences the DNA methylation of subsequent siblings. We measured whole blood methylation using the HumanMethylation450 array for siblings from two twin and family studies in Australia and Korea. We compared the means and correlations in methylation between pairs of siblings born before a twin birth (BT siblings), born on either side of a twin birth (B/AT pairs) and born after a twin birth (AT siblings). For the genome-wide average DNA methylation, the correlation for AT pairs (rAT) was larger than the correlation for BT pairs (rBT) in both studies, and from the meta-analysis, rAT = 0.46 (95% CI: 0.26, 0.63) and rBT = -0.003 (95% CI: -0.30, 0.29) (P = 0.02). B/AT pairs were not correlated (from the meta-analysis rBAT = 0.08; 95% CI: -0.31, 0.45). Similar results were found for the average methylation of several genomic regions, e.g., CpG shelf and gene body. BT and AT pairs were differentially correlated in methylation for 15 probes (all P < 10-7), and the top 152 differentially correlated probes (at P < 10-4) were enriched in cell signalling and breast cancer regulation pathways. Our observations are consistent with a twin birth changing the intrauterine environment such that siblings both born after a twin birth are correlated in DNA methylation.

LanguageEnglish
Article number8463
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - 1 Dec 2017

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DNA Methylation
Parturition
Methylation
Meta-Analysis
Twin Studies
Korea
Genome
Breast Neoplasms
Genes

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Li, S., Kim, E., Wong, E. M., Joo, J. H. E., Nguyen, T. L., Stone, J., ... Hopper, J. L. (2017). Twin birth changes DNA methylation of subsequent siblings. Scientific Reports, 7(1), [8463]. DOI: 10.1038/s41598-017-08595-6
Li, Shuai ; Kim, Eunae ; Wong, Ee Ming ; Joo, Ji Hoon Eric ; Nguyen, Tuong L. ; Stone, Jennifer ; Song, Yun Mi ; Flander, Louisa B. ; Saffery, Richard ; Giles, Graham G. ; Southey, Melissa C. ; Sung, Joohon ; Hopper, John L./ Twin birth changes DNA methylation of subsequent siblings. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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Li, S, Kim, E, Wong, EM, Joo, JHE, Nguyen, TL, Stone, J, Song, YM, Flander, LB, Saffery, R, Giles, GG, Southey, MC, Sung, J & Hopper, JL 2017, 'Twin birth changes DNA methylation of subsequent siblings' Scientific Reports, vol 7, no. 1, 8463. DOI: 10.1038/s41598-017-08595-6

Twin birth changes DNA methylation of subsequent siblings. / Li, Shuai; Kim, Eunae; Wong, Ee Ming; Joo, Ji Hoon Eric; Nguyen, Tuong L.; Stone, Jennifer; Song, Yun Mi; Flander, Louisa B.; Saffery, Richard; Giles, Graham G.; Southey, Melissa C.; Sung, Joohon; Hopper, John L.

In: Scientific Reports, Vol. 7, No. 1, 8463, 01.12.2017.

Research output: Contribution to journalArticle

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AU - Saffery,Richard

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Li S, Kim E, Wong EM, Joo JHE, Nguyen TL, Stone J et al. Twin birth changes DNA methylation of subsequent siblings. Scientific Reports. 2017 Dec 1;7(1). 8463. Available from, DOI: 10.1038/s41598-017-08595-6