TT virus: Preferential distribution in CD19(+) peripheral blood mononuclear cells and lack of viral integration

Q.G. Yu, B. Shiramizu, V.R. Nerurkar, N.J. Hu, CM Shikuma, M.E. Melish, K Cascio, A Imrie, Y. Lu, R. Yanagihara

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Abstract

In order to determine whether peripheral blood mononuclear cells (PBMC) contain proviral DNA or double-stranded replicative forms of TT virus (TTV) and to define which cell subset harbors TTV, we analyzed 126 PBMC DNA samples by PCR, using the NG059/NG061/NG063 and the T801/T935 primer sets. TTV sequences were detected in nearly 20%(25/126) of PBMC DNA samples. Analysis of PBMC subsets revealed that TTV was preferentially distributed in CD19(+) cells. TTV DNA was also detected in CD3(+)/CD19(+) double-depleted cells but was undetectable in CD3(+) cells. After degradation of single-stranded DNA using mung bean nuclease (MBN), TTV could not be detected in previously TTV-positive PBMC DNA samples nor in DNA from CD19+ cells, CD3(+)-depleted, and CD3(+)/CD19(+) double-depleted cells, indicating the lack of TTV DNA integration and the absence of viral double-stranded replicative intermediates in PBMC or PBMC subset cells. Our data indicate that PBMC and PBMC subsets are not the major sites of TTV replication. J. Med. Virol. 66:276-284, 2002. (C) 2002 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)276-284
Number of pages9
JournalJournal of Medical Virology
Volume66
Issue number2
DOIs
Publication statusPublished - Feb 2002

Cite this

Yu, Q. G., Shiramizu, B., Nerurkar, V. R., Hu, N. J., Shikuma, CM., Melish, M. E., Cascio, K., Imrie, A., Lu, Y., & Yanagihara, R. (2002). TT virus: Preferential distribution in CD19(+) peripheral blood mononuclear cells and lack of viral integration. Journal of Medical Virology, 66(2), 276-284. https://doi.org/10.1002/jmv.2142