Transmission of two Australian strains of murine cytomegalovirus (MCMV) in enclosure populations of house mice (Mus domesticus)

L.N. Farroway, Shelley Gorman, Malcolm Lawson, N.L. Harvey, D.A. Jones, Geoffrey Shellam, G.R. Singleton

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

To control plagues of free-living mice (Mus domesticus) in Australia, a recombinant murine cytomegalovirus (MCMV) expressing fertility proteins is being developed as an immunocontraceptive agent. Real-time quantitative PCR was used to monitor the transmission of two genetically variable field strains of MCMV through mouse populations after 25 % of founding mice were infected with the N1 strain, followed by the G4 strain 6 weeks later. Pathogen-free wild-derived mice were released into outdoor enclosures located in northwestern Victoria (Australia). Of those mice not originally inoculated with virus, NI DNA was detected in more than 80 % of founder mice and a third of their offspring and similarly, G4 DNA was detected in 13 % of founder mice and in 3 % of their offspring. Thus, prior immunity to NI did not prevent transmission of G4. This result is promising for successful transmission of an immunocontraceptive vaccine through Australian mouse populations where MCMV infection is endemic.
Original languageEnglish
Pages (from-to)701-710
JournalEpidemiology and Infection
Volume133
Issue number4
DOIs
Publication statusPublished - 2005

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Muromegalovirus
Population
DNA Viruses
Plague
Victoria
Cytomegalovirus Infections
Fertility
Real-Time Polymerase Chain Reaction
Immunity
Vaccines

Cite this

Farroway, L.N. ; Gorman, Shelley ; Lawson, Malcolm ; Harvey, N.L. ; Jones, D.A. ; Shellam, Geoffrey ; Singleton, G.R. / Transmission of two Australian strains of murine cytomegalovirus (MCMV) in enclosure populations of house mice (Mus domesticus). In: Epidemiology and Infection. 2005 ; Vol. 133, No. 4. pp. 701-710.
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Transmission of two Australian strains of murine cytomegalovirus (MCMV) in enclosure populations of house mice (Mus domesticus). / Farroway, L.N.; Gorman, Shelley; Lawson, Malcolm; Harvey, N.L.; Jones, D.A.; Shellam, Geoffrey; Singleton, G.R.

In: Epidemiology and Infection, Vol. 133, No. 4, 2005, p. 701-710.

Research output: Contribution to journalArticle

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AB - To control plagues of free-living mice (Mus domesticus) in Australia, a recombinant murine cytomegalovirus (MCMV) expressing fertility proteins is being developed as an immunocontraceptive agent. Real-time quantitative PCR was used to monitor the transmission of two genetically variable field strains of MCMV through mouse populations after 25 % of founding mice were infected with the N1 strain, followed by the G4 strain 6 weeks later. Pathogen-free wild-derived mice were released into outdoor enclosures located in northwestern Victoria (Australia). Of those mice not originally inoculated with virus, NI DNA was detected in more than 80 % of founder mice and a third of their offspring and similarly, G4 DNA was detected in 13 % of founder mice and in 3 % of their offspring. Thus, prior immunity to NI did not prevent transmission of G4. This result is promising for successful transmission of an immunocontraceptive vaccine through Australian mouse populations where MCMV infection is endemic.

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