Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 327-334 |
| Journal | Journal of Hepatology |
| Volume | 48 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 |
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Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron. / Graham, Ross; Reutens, G.M.; Herbison, C.E.; Delima, R.D.; Chua, A.C.G.; Olynyk, John; Trinder, Debbie.
In: Journal of Hepatology, Vol. 48, No. 2, 2008, p. 327-334.Research output: Contribution to journal › Article
TY - JOUR
T1 - Transferrin receptor 2 mediates uptake of transferrin-bound and non-transferrin-bound iron
AU - Graham, Ross
AU - Reutens, G.M.
AU - Herbison, C.E.
AU - Delima, R.D.
AU - Chua, A.C.G.
AU - Olynyk, John
AU - Trinder, Debbie
PY - 2008
Y1 - 2008
N2 - Background/Aims: Transferrin receptor 2 appears to have dual roles in iron metabolism; one is signalling, the other is iron transport. It is sensitive to high levels of diferric transferrin, which is associated with disorders of iron overload. Also present in these disorders are increased levels of plasma non-transferrin-bound iron. This study sought to clarify the role of transferrin receptor 2 in the uptake of transferrin-bound and non-transferrin-bound iron. Methods: Variant Chinese Hamster Ovary (CHO) cells, transfected with transferrin receptor 2, were incubated with radio-labelled transferrin-bound or non-transferrin-bound iron. Competition studies were performed in the presence of unlabelled dimetallic transferrin; knockdown was performed using specific siRNA. Results :Cells expressing transferrin receptor 2 bound and internalised transferrin and transferrin-bound iron. Transferrin recycling occurred with an average cycling time of 11–15 min. Interestingly, the presence of transferrin receptor 2 was also associated with uptake of non-transferrin-bound iron which was inhibited by unlabelled transferrin-bound metals. Knockdown reduced transferrin-bound and non-transferrin-bound iron uptake by approximately 60%. Conclusions: Transferrin receptor 2 mediates transferrin-bound iron uptake by receptor-mediated endocytosis. It is also involved in the uptake of non-transferrin-bound iron and the inhibition of non-transferrin-bound iron uptake by diferric transferrin in CHO cells.
AB - Background/Aims: Transferrin receptor 2 appears to have dual roles in iron metabolism; one is signalling, the other is iron transport. It is sensitive to high levels of diferric transferrin, which is associated with disorders of iron overload. Also present in these disorders are increased levels of plasma non-transferrin-bound iron. This study sought to clarify the role of transferrin receptor 2 in the uptake of transferrin-bound and non-transferrin-bound iron. Methods: Variant Chinese Hamster Ovary (CHO) cells, transfected with transferrin receptor 2, were incubated with radio-labelled transferrin-bound or non-transferrin-bound iron. Competition studies were performed in the presence of unlabelled dimetallic transferrin; knockdown was performed using specific siRNA. Results :Cells expressing transferrin receptor 2 bound and internalised transferrin and transferrin-bound iron. Transferrin recycling occurred with an average cycling time of 11–15 min. Interestingly, the presence of transferrin receptor 2 was also associated with uptake of non-transferrin-bound iron which was inhibited by unlabelled transferrin-bound metals. Knockdown reduced transferrin-bound and non-transferrin-bound iron uptake by approximately 60%. Conclusions: Transferrin receptor 2 mediates transferrin-bound iron uptake by receptor-mediated endocytosis. It is also involved in the uptake of non-transferrin-bound iron and the inhibition of non-transferrin-bound iron uptake by diferric transferrin in CHO cells.
U2 - 10.1016/j.jhep.2007.10.009
DO - 10.1016/j.jhep.2007.10.009
M3 - Article
VL - 48
SP - 327
EP - 334
JO - Journal of Hepatology
JF - Journal of Hepatology
SN - 0168-8278
IS - 2
ER -