Abstract
Cellular transitions are essential to tissue repair, cell differentiation, and maintaining tissue function. Single-cell sequencing can identify subtle differences in molecular features including gene expression, protein levels, chromatin architecture, and epigenetic modifications that result in different cell states. Non-canonical DNA secondary structures are highly dynamic and play important roles in regulating these molecular features. This thesis investigates transcriptional alterations, and the roleof two non-canonical DNA secondary structures, G-quadruplexes and i-motifs, in regulating cellulartransitions. To achieve this, an antigen-independent multiplex single-cell RNA sequencing methodology was developed and implemented.
Original language | English |
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Qualification | Doctor of Philosophy |
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Award date | 12 Jan 2024 |
DOIs | |
Publication status | Unpublished - 2023 |