Transfection based single-cell sequencing to probe cellular states and spatial heterogeneity

Research output: ThesisDoctoral Thesis

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Abstract

Cellular transitions are essential to tissue repair, cell differentiation, and maintaining tissue function. Single-cell sequencing can identify subtle differences in molecular features including gene expression, protein levels, chromatin architecture, and epigenetic modifications that result in different cell states. Non-canonical DNA secondary structures are highly dynamic and play important roles in regulating these molecular features. This thesis investigates transcriptional alterations, and the roleof two non-canonical DNA secondary structures, G-quadruplexes and i-motifs, in regulating cellulartransitions. To achieve this, an antigen-independent multiplex single-cell RNA sequencing methodology was developed and implemented.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Smith, Nicole, Supervisor
  • Swaminatha Iyer, Iyer, Supervisor
  • Evans, Cameron, Supervisor
Thesis sponsors
Award date12 Jan 2024
DOIs
Publication statusUnpublished - 2023

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