TY - JOUR
T1 - Transcriptomic profiling of near-isogenic lines reveals candidate genes for a significant locus conferring metribuzin resistance in wheat
AU - Bhattarai, Rudra
AU - Liu, Hui
AU - Siddique, Kadambot H.M.
AU - Yan, Guijun
N1 - Funding Information:
This research was funded by the Global Innovation Linkages Project (GIL53853) from the Australian Department of Industry, Science and Resources, and The University of Western Australia.
Funding Information:
We would like to thank the Bioinformatics Center, Institute for Chemical Research, Kyoto University and Human Genome Center, Institute of Medical Science, University of Tokyo for developing KEGG software, and Kanehisa laboratories for providing the permission to publish under the CC BY 4.0 open-access license of KEGG pathway map images in this manuscript. We also thank Mrs. Anita Severn-Ellis for her support in molecular lab availability and induction and Mr. Greg Cawthray for technical support in operations of the Geno-grinder. We thank Novogene Singapore for next-generation sequencing services. The whole manuscript has been revised and edited by a professional English language editor, Dr Chris Davies of Tweak Editing.
Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12
Y1 - 2023/12
N2 - Background: Weeds reduce wheat yields in dryland farming systems. Herbicides such as metribuzin are commonly used to control weeds. However, wheat has a narrow safety margin against metribuzin. Standing crops such as wheat with weeds in the same field can also be killed by the same dose of metribuzin. Therefore, it is important to identify metribuzin resistance genes and understand the resistance mechanism in wheat for sustainable crop production. A previous study identified a significant metribuzin resistance wheat QTL, Qsns.uwa.4 A.2, explaining 69% of the phenotypic variance for metribuzin resistance. Results: Two NIL pairs with the most contrasting performance in the metribuzin treatment and different in genetic backgrounds were compared using RNA sequence analysis, identifying nine candidate genes underlying Qsns.uwa.4 A.2 responsible for metribuzin resistance. Quantitative RT-qPCR further validated the candidate genes, with TraesCS4A03G1099000 (nitrate excretion transporter), TraesCS4A03G1181300 (aspartyl protease), and TraesCS4A03G0741300 (glycine-rich proteins) identified as key factors for metribuzin resistance. Conclusion: Identified markers and key candidate genes can be used for selecting metribuzin resistance in wheat.
AB - Background: Weeds reduce wheat yields in dryland farming systems. Herbicides such as metribuzin are commonly used to control weeds. However, wheat has a narrow safety margin against metribuzin. Standing crops such as wheat with weeds in the same field can also be killed by the same dose of metribuzin. Therefore, it is important to identify metribuzin resistance genes and understand the resistance mechanism in wheat for sustainable crop production. A previous study identified a significant metribuzin resistance wheat QTL, Qsns.uwa.4 A.2, explaining 69% of the phenotypic variance for metribuzin resistance. Results: Two NIL pairs with the most contrasting performance in the metribuzin treatment and different in genetic backgrounds were compared using RNA sequence analysis, identifying nine candidate genes underlying Qsns.uwa.4 A.2 responsible for metribuzin resistance. Quantitative RT-qPCR further validated the candidate genes, with TraesCS4A03G1099000 (nitrate excretion transporter), TraesCS4A03G1181300 (aspartyl protease), and TraesCS4A03G0741300 (glycine-rich proteins) identified as key factors for metribuzin resistance. Conclusion: Identified markers and key candidate genes can be used for selecting metribuzin resistance in wheat.
KW - Differential gene expression
KW - Marker-assisted selection
KW - Metribuzin resistance
KW - Near-isogenic lines
KW - Wheat
UR - http://www.scopus.com/inward/record.url?scp=85158122006&partnerID=8YFLogxK
U2 - 10.1186/s12870-023-04166-2
DO - 10.1186/s12870-023-04166-2
M3 - Article
C2 - 37142987
AN - SCOPUS:85158122006
SN - 1471-2229
VL - 23
JO - BMC Plant Biology
JF - BMC Plant Biology
IS - 1
M1 - 237
ER -