Transcriptomic analysis of patient plasma reveals circulating miR200c as a potential biomarker for high-grade serous ovarian cancer

Natalie J Hannan, Paul A Cohen, Sally Beard, Sanela Bilic, Bonnie Zhang, Stephen Tong, Clare Whitehead, Lisa Hui

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Abstract

Background High-grade serous tubo-ovarian cancer (HGSC) is the most common histological subtype of epithelial ovarian cancer, and highly lethal. Currently there is no effective screening test and prognosis is poor as the majority of cases are diagnosed at the advanced stage. Cell free RNAs including microRNAs (miRNAs) are dysregulated in ovarian cancer tissue and are detectable in the circulation. This study aimed to determine whether circulating cell free miRNAs may be potential biomarkers for the detection of HGSC. Methods Plasma was collected from women with HGSC (Grade 3, n=24), and benign ovarian masses (n=24). RNA was extracted from patient plasma and subjected to miRNA targeted next generation sequencing (NGS). A subsequent validation cohort was assessed using plasma collected from women with HGSC (n=14) and controls (with a benign ovarian mass; n=15). RNA was extracted and assessed using quantitative RT-PCR. Results Differential gene expression (DGE) of the NGS data revealed a significant increase in the miRNA, miR200c, in the circulation of women with HGSC (p
Original languageEnglish
Article number100894
JournalGynecologic Oncology Reports
Volume39
DOIs
Publication statusPublished - Feb 2022

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