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Transcript-Based Detection of Circulating Melanoma Cells

Research output: Chapter in Book/Conference paperChapterpeer-review

Abstract

Circulating tumor cells (CTCs) are cancer cells shed by the primary tumor or its metastases that circulate in the peripheral blood. CTCs are potential seeds for metastases, and their detection may allow early uncovering of metastatic dissemination and disease prognostication. To fully ascertain the biomarker potential of melanoma CTCs, sensitive and reliable methods are required. Melanoma-specific transcript analysis has been widely utilized as a standard approach for measuring the presence of CTCs. Here we describe a method for the analysis of CTCs through the detection of specific transcripts in CTC-enriched fractions. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.
Original languageEnglish
Title of host publicationMelanoma
Subtitle of host publicationMethods and Protocols
EditorsKristian M. Hargadon
PublisherSpringer
Chapter18
Pages235-245
Number of pages11
ISBN (Electronic)978-1-0716-1205-7, 978-1-0716-1204-0
ISBN (Print)978-1-0716-1207-1
DOIs
Publication statusPublished - 2021
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2265
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

  1. SDG 3 - Good Health and Well-being
    SDG 3 Good Health and Well-being

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