Tracing B-genome chromatin in Brassica napus x B. juncea interspecific progeny

C.J. Schelfhout, R. Snowdon, Wallace Cowling, Janet Wroth

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We used polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques to demonstrate the presence of Brassica B-genome chromosomes and putative B-genome introgressions in B. napus x B. juncea interspecific progeny., The B-genome - specific repeat sequence pBNBH35 was used to generate PCR products and FISH probes. The highest frequencies of viable progeny were obtained when B. napus was the maternal parent of the interspecific hybrid and the first backcross. B-genome - positive PCR assays were found in 34/51 fertile F-2 progeny (67%), which was more than double the proportion found in fertile BC, progeny. Four B-genome - positive F-2-derived families and I BC1-derived family were fixed or segregating for B. juncea morphology in the F-4 and BC1S2, respectively, but in only 2 of these families did B. juncea-type plants exhibit B. juncea chromosome count (2n = 36) and typical B-genome FISH signals on 16 chromosomes. The remaining B. juncea-type plants had B. napus chromosome count (2n = 38) and no B-genome FISH signals, except for 1 exceptional F-4-derived line that exhibited isolated and weak B-genome FISH signals on 11 chromosomes and typical A-genome FISH signals. B. juncea morphology was associated with B-genome - positive PCR signals but not necessarily with 16 intact B-genome chromosomes as detected by FISH. B-genome chromosomes tend to be eliminated during selfing or backcrossing after crossing B. juncea with B. napus, and selection of lines containing B-genome chromatin during early generations would be promoted by use of this B-genome repetitive marker.
Original languageEnglish
Pages (from-to)1490-1497
JournalGenome
Volume49
Issue number11
DOIs
Publication statusPublished - 2006

Fingerprint

Brassica napus
Brassica juncea
Chromatin
chromatin
Genome
genome
fluorescence in situ hybridization
Fluorescence In Situ Hybridization
Chromosomes, Human, 4-5
chromosomes
polymerase chain reaction
Polymerase Chain Reaction
chromosome number
Chromosomes, Human, Pair 16
Chromosomes, Human, Pair 11
Inbreeding
backcrossing
Brassica
selfing
introgression

Cite this

Schelfhout, C.J. ; Snowdon, R. ; Cowling, Wallace ; Wroth, Janet. / Tracing B-genome chromatin in Brassica napus x B. juncea interspecific progeny. In: Genome. 2006 ; Vol. 49, No. 11. pp. 1490-1497.
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abstract = "We used polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques to demonstrate the presence of Brassica B-genome chromosomes and putative B-genome introgressions in B. napus x B. juncea interspecific progeny., The B-genome - specific repeat sequence pBNBH35 was used to generate PCR products and FISH probes. The highest frequencies of viable progeny were obtained when B. napus was the maternal parent of the interspecific hybrid and the first backcross. B-genome - positive PCR assays were found in 34/51 fertile F-2 progeny (67{\%}), which was more than double the proportion found in fertile BC, progeny. Four B-genome - positive F-2-derived families and I BC1-derived family were fixed or segregating for B. juncea morphology in the F-4 and BC1S2, respectively, but in only 2 of these families did B. juncea-type plants exhibit B. juncea chromosome count (2n = 36) and typical B-genome FISH signals on 16 chromosomes. The remaining B. juncea-type plants had B. napus chromosome count (2n = 38) and no B-genome FISH signals, except for 1 exceptional F-4-derived line that exhibited isolated and weak B-genome FISH signals on 11 chromosomes and typical A-genome FISH signals. B. juncea morphology was associated with B-genome - positive PCR signals but not necessarily with 16 intact B-genome chromosomes as detected by FISH. B-genome chromosomes tend to be eliminated during selfing or backcrossing after crossing B. juncea with B. napus, and selection of lines containing B-genome chromatin during early generations would be promoted by use of this B-genome repetitive marker.",
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Tracing B-genome chromatin in Brassica napus x B. juncea interspecific progeny. / Schelfhout, C.J.; Snowdon, R.; Cowling, Wallace; Wroth, Janet.

In: Genome, Vol. 49, No. 11, 2006, p. 1490-1497.

Research output: Contribution to journalArticle

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AB - We used polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH) techniques to demonstrate the presence of Brassica B-genome chromosomes and putative B-genome introgressions in B. napus x B. juncea interspecific progeny., The B-genome - specific repeat sequence pBNBH35 was used to generate PCR products and FISH probes. The highest frequencies of viable progeny were obtained when B. napus was the maternal parent of the interspecific hybrid and the first backcross. B-genome - positive PCR assays were found in 34/51 fertile F-2 progeny (67%), which was more than double the proportion found in fertile BC, progeny. Four B-genome - positive F-2-derived families and I BC1-derived family were fixed or segregating for B. juncea morphology in the F-4 and BC1S2, respectively, but in only 2 of these families did B. juncea-type plants exhibit B. juncea chromosome count (2n = 36) and typical B-genome FISH signals on 16 chromosomes. The remaining B. juncea-type plants had B. napus chromosome count (2n = 38) and no B-genome FISH signals, except for 1 exceptional F-4-derived line that exhibited isolated and weak B-genome FISH signals on 11 chromosomes and typical A-genome FISH signals. B. juncea morphology was associated with B-genome - positive PCR signals but not necessarily with 16 intact B-genome chromosomes as detected by FISH. B-genome chromosomes tend to be eliminated during selfing or backcrossing after crossing B. juncea with B. napus, and selection of lines containing B-genome chromatin during early generations would be promoted by use of this B-genome repetitive marker.

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