Topically applied 1,25-dihydroxyvitamin D3 enhances the suppressive activity of CD4+CD25+ cells in the draining lymph nodes

Shelley Gorman, L.A. Kuritzky, M.A. Judge, K.M. Dixon, J.P. Mcglade, R.S. Mason, John Finlay-Jones, Prudence Hart

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

The immunomodulatory effects of vitamin D have been described following chronic oral administration to mice or supplementation of cell cultures with 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3)1 the active form of vitamin D. In this study, topically applied 1,25(OH)(2)D-3, enhanced the suppressive capacity of CD4(+)CD25(+) cells from the draining lymph nodes. The effects of topical 1-25(OH)(2)D-3 were compared with those of UVB irradiation, which is the environmental factor required for 1,25(OH)(2)D-3 production in skin. CD4(+) cells from the skin-draining lymph nodes (SDLN) of either 1,25(OH)(2)D-3-treated or UVB-irradiated mice had reduced capacity to proliferate to Ags presented in vitro, and could suppress Ag-specific immune responses upon adoptive transfer into naive mice. This regulation was lost upon removal of CD4(+)CD25(+) cells. Furthermore, purified CD4(+)CD25(+) cells from the SDLN of 1,25(OH)(2)D-3-treated or UVB-irradiated mice compared with equal numbers of CD4(+)CD25(+) cells from control mice had increased capacity to suppress immune responses in both in vitro and in vivo assay systems. Following the sensitization of recipient mice with OVA, the proportion of CD4(+)Foxp3(+) cells of donor origin significantly increased in recipients of CD4(+)CD25(+) cells from the SDLN of 1,25(OH)2D3-treated mice, indicating that these regulatory T cells can expand in vivo with antigenic stimulation. These studies suggest that 1,25(OH)(2)D-3 may be an important mediator by which UVB-irradiation exerts some of its immunomodulatory effects.
Original languageEnglish
Pages (from-to)6273-6283
JournalJournal of Immunology
Volume179
Issue number9
DOIs
Publication statusPublished - 2007

Fingerprint

Calcitriol
Lymph Nodes
Skin
Vitamin D
Adoptive Transfer
Regulatory T-Lymphocytes
Oral Administration
Cell Culture Techniques

Cite this

Gorman, Shelley ; Kuritzky, L.A. ; Judge, M.A. ; Dixon, K.M. ; Mcglade, J.P. ; Mason, R.S. ; Finlay-Jones, John ; Hart, Prudence. / Topically applied 1,25-dihydroxyvitamin D3 enhances the suppressive activity of CD4+CD25+ cells in the draining lymph nodes. In: Journal of Immunology. 2007 ; Vol. 179, No. 9. pp. 6273-6283.
@article{cfb505bee9e74049ab320cecfbc68457,
title = "Topically applied 1,25-dihydroxyvitamin D3 enhances the suppressive activity of CD4+CD25+ cells in the draining lymph nodes",
abstract = "The immunomodulatory effects of vitamin D have been described following chronic oral administration to mice or supplementation of cell cultures with 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3)1 the active form of vitamin D. In this study, topically applied 1,25(OH)(2)D-3, enhanced the suppressive capacity of CD4(+)CD25(+) cells from the draining lymph nodes. The effects of topical 1-25(OH)(2)D-3 were compared with those of UVB irradiation, which is the environmental factor required for 1,25(OH)(2)D-3 production in skin. CD4(+) cells from the skin-draining lymph nodes (SDLN) of either 1,25(OH)(2)D-3-treated or UVB-irradiated mice had reduced capacity to proliferate to Ags presented in vitro, and could suppress Ag-specific immune responses upon adoptive transfer into naive mice. This regulation was lost upon removal of CD4(+)CD25(+) cells. Furthermore, purified CD4(+)CD25(+) cells from the SDLN of 1,25(OH)(2)D-3-treated or UVB-irradiated mice compared with equal numbers of CD4(+)CD25(+) cells from control mice had increased capacity to suppress immune responses in both in vitro and in vivo assay systems. Following the sensitization of recipient mice with OVA, the proportion of CD4(+)Foxp3(+) cells of donor origin significantly increased in recipients of CD4(+)CD25(+) cells from the SDLN of 1,25(OH)2D3-treated mice, indicating that these regulatory T cells can expand in vivo with antigenic stimulation. These studies suggest that 1,25(OH)(2)D-3 may be an important mediator by which UVB-irradiation exerts some of its immunomodulatory effects.",
author = "Shelley Gorman and L.A. Kuritzky and M.A. Judge and K.M. Dixon and J.P. Mcglade and R.S. Mason and John Finlay-Jones and Prudence Hart",
year = "2007",
doi = "10.4049/jimmunol.179.9.6273",
language = "English",
volume = "179",
pages = "6273--6283",
journal = "The Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",

}

Topically applied 1,25-dihydroxyvitamin D3 enhances the suppressive activity of CD4+CD25+ cells in the draining lymph nodes. / Gorman, Shelley; Kuritzky, L.A.; Judge, M.A.; Dixon, K.M.; Mcglade, J.P.; Mason, R.S.; Finlay-Jones, John; Hart, Prudence.

In: Journal of Immunology, Vol. 179, No. 9, 2007, p. 6273-6283.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Topically applied 1,25-dihydroxyvitamin D3 enhances the suppressive activity of CD4+CD25+ cells in the draining lymph nodes

AU - Gorman, Shelley

AU - Kuritzky, L.A.

AU - Judge, M.A.

AU - Dixon, K.M.

AU - Mcglade, J.P.

AU - Mason, R.S.

AU - Finlay-Jones, John

AU - Hart, Prudence

PY - 2007

Y1 - 2007

N2 - The immunomodulatory effects of vitamin D have been described following chronic oral administration to mice or supplementation of cell cultures with 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3)1 the active form of vitamin D. In this study, topically applied 1,25(OH)(2)D-3, enhanced the suppressive capacity of CD4(+)CD25(+) cells from the draining lymph nodes. The effects of topical 1-25(OH)(2)D-3 were compared with those of UVB irradiation, which is the environmental factor required for 1,25(OH)(2)D-3 production in skin. CD4(+) cells from the skin-draining lymph nodes (SDLN) of either 1,25(OH)(2)D-3-treated or UVB-irradiated mice had reduced capacity to proliferate to Ags presented in vitro, and could suppress Ag-specific immune responses upon adoptive transfer into naive mice. This regulation was lost upon removal of CD4(+)CD25(+) cells. Furthermore, purified CD4(+)CD25(+) cells from the SDLN of 1,25(OH)(2)D-3-treated or UVB-irradiated mice compared with equal numbers of CD4(+)CD25(+) cells from control mice had increased capacity to suppress immune responses in both in vitro and in vivo assay systems. Following the sensitization of recipient mice with OVA, the proportion of CD4(+)Foxp3(+) cells of donor origin significantly increased in recipients of CD4(+)CD25(+) cells from the SDLN of 1,25(OH)2D3-treated mice, indicating that these regulatory T cells can expand in vivo with antigenic stimulation. These studies suggest that 1,25(OH)(2)D-3 may be an important mediator by which UVB-irradiation exerts some of its immunomodulatory effects.

AB - The immunomodulatory effects of vitamin D have been described following chronic oral administration to mice or supplementation of cell cultures with 1,25-dihydroxyvitamin D-3 (1,25(OH)(2)D-3)1 the active form of vitamin D. In this study, topically applied 1,25(OH)(2)D-3, enhanced the suppressive capacity of CD4(+)CD25(+) cells from the draining lymph nodes. The effects of topical 1-25(OH)(2)D-3 were compared with those of UVB irradiation, which is the environmental factor required for 1,25(OH)(2)D-3 production in skin. CD4(+) cells from the skin-draining lymph nodes (SDLN) of either 1,25(OH)(2)D-3-treated or UVB-irradiated mice had reduced capacity to proliferate to Ags presented in vitro, and could suppress Ag-specific immune responses upon adoptive transfer into naive mice. This regulation was lost upon removal of CD4(+)CD25(+) cells. Furthermore, purified CD4(+)CD25(+) cells from the SDLN of 1,25(OH)(2)D-3-treated or UVB-irradiated mice compared with equal numbers of CD4(+)CD25(+) cells from control mice had increased capacity to suppress immune responses in both in vitro and in vivo assay systems. Following the sensitization of recipient mice with OVA, the proportion of CD4(+)Foxp3(+) cells of donor origin significantly increased in recipients of CD4(+)CD25(+) cells from the SDLN of 1,25(OH)2D3-treated mice, indicating that these regulatory T cells can expand in vivo with antigenic stimulation. These studies suggest that 1,25(OH)(2)D-3 may be an important mediator by which UVB-irradiation exerts some of its immunomodulatory effects.

U2 - 10.4049/jimmunol.179.9.6273

DO - 10.4049/jimmunol.179.9.6273

M3 - Article

VL - 179

SP - 6273

EP - 6283

JO - The Journal of Immunology

JF - The Journal of Immunology

SN - 0022-1767

IS - 9

ER -