Three-dimensional mechanical characterization of murine skeletal muscle using quantitative micro-elastography

Erin M. Lloyd, Matt S. Hepburn, Jiayue Li, Alireza Mowla, Yongsung Hwang, Yu Suk Choi, Miranda D. Grounds, Brendan F. Kennedy

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


Skeletal muscle function is governed by both the mechanical and structural propertiesof its constituent tissues, which are both modified by disease. Characterizing the mechanicalproperties of skeletal muscle tissue at an intermediate scale, i.e., between that of cells and organs, can provide insight into diseases such as muscular dystrophies. In this study, we use quantitative micro-elastography (QME) to characterize the micro-scale elasticity of ex vivo murine skeletal muscle in three-dimensions in whole muscles. To address the challenge of achieving high QME image quality with samples featuring uneven surfaces and geometry, we encapsulate the muscles in transparent hydrogels with flat surfaces. Using this method, we study aging and disease in quadriceps tissue by comparing normal wild-type (C57BL/6J) mice with dysferlin-deficient BLAJ mice, a model for the muscular dystrophy dysferlinopathy, at 3, 10, and 24 months of age (sample size of three per group). We observe a 77% decrease in elasticity at 24 months in dysferlin-deficient quadriceps compared to wild-type quadriceps.
Original languageEnglish
Article number5879
Pages (from-to)5879-5899
Number of pages21
JournalBiomedical Optics Express
Issue number11
Publication statusPublished - Nov 2022


Dive into the research topics of 'Three-dimensional mechanical characterization of murine skeletal muscle using quantitative micro-elastography'. Together they form a unique fingerprint.

Cite this