Projects per year
Abstract
Skeletal muscle function is governed by both the mechanical and structural propertiesof its constituent tissues, which are both modified by disease. Characterizing the mechanicalproperties of skeletal muscle tissue at an intermediate scale, i.e., between that of cells and organs, can provide insight into diseases such as muscular dystrophies. In this study, we use quantitative micro-elastography (QME) to characterize the micro-scale elasticity of ex vivo murine skeletal muscle in three-dimensions in whole muscles. To address the challenge of achieving high QME image quality with samples featuring uneven surfaces and geometry, we encapsulate the muscles in transparent hydrogels with flat surfaces. Using this method, we study aging and disease in quadriceps tissue by comparing normal wild-type (C57BL/6J) mice with dysferlin-deficient BLAJ mice, a model for the muscular dystrophy dysferlinopathy, at 3, 10, and 24 months of age (sample size of three per group). We observe a 77% decrease in elasticity at 24 months in dysferlin-deficient quadriceps compared to wild-type quadriceps.
Original language | English |
---|---|
Article number | 5879 |
Pages (from-to) | 5879-5899 |
Number of pages | 21 |
Journal | Biomedical Optics Express |
Volume | 13 |
Issue number | 11 |
DOIs | |
Publication status | Published - Nov 2022 |
Fingerprint
Dive into the research topics of 'Three-dimensional mechanical characterization of murine skeletal muscle using quantitative micro-elastography'. Together they form a unique fingerprint.Projects
- 1 Finished
-
Intravital mechano-microscopy: a discovery platform for cell mechanics
Kennedy, B., Grounds, M., Munro, P. & Pelling, A.
ARC Australian Research Council
1/01/16 → 31/12/20
Project: Research
Research output
- 3 Citations
- 1 Doctoral Thesis
-
Development of optical coherence elastography towards precise and accurate mechanical characterisation of cells and tissues
Li, J., 2023, (Unpublished)Research output: Thesis › Doctoral Thesis
File176 Downloads (Pure)