Thiol-Disulfide Redox Proteomics in Plant Research

Meenakumari Muthuramalingam, Karl-Josef Dietz, Elke Stroeher

Research output: Chapter in Book/Conference paperChapter

6 Citations (Scopus)

Abstract

Abiotic stresses often cause metabolic imbalances which affect cellular redox homeostasis and alter the rate of reduction state of functional and regulatory protein thiols and the rate of reactive oxygen species release. Excessive displacement from redox equilibrium causes oxidative damage to cell structures and may elicit cell death. The understanding of the cell response to progressive stress must include knowledge on the thiol redox state of specific proteins. This chapter describes selected gel-based biochemical methods (i) to identify thiol disulfide redox proteins that undergo major redox-dependent conformational changes by 2D redox SDS-PAGE and (ii) to determine the thiol redox state of proteins by sequential blocking and labeling with N-ethylmaleimide and methoxypolyethylene glycol maleimide-5000 (mPEG-Mal-5000). Both sets of methods provide experimental information that defines the redox proteome of the cell.

Original languageEnglish
Title of host publicationPLANT STRESS TOLERANCE: METHODS AND PROTOCOLS
EditorsR Sunkar
PublisherHumana Press
Pages219-238
Number of pages20
ISBN (Print)978-1-60761-701-3
DOIs
Publication statusPublished - 2010
Externally publishedYes

Publication series

NameMethods in Molecular Biology
PublisherHUMANA PRESS INC
Volume639
ISSN (Print)1064-3745

Cite this

Muthuramalingam, M., Dietz, K-J., & Stroeher, E. (2010). Thiol-Disulfide Redox Proteomics in Plant Research. In R. Sunkar (Ed.), PLANT STRESS TOLERANCE: METHODS AND PROTOCOLS (pp. 219-238). (Methods in Molecular Biology; Vol. 639). Humana Press. https://doi.org/10.1007/978-1-60761-702-0_13