Thermal Trauma and the Effect of Temperature and Time on the Morphology and DNA of Dentition

Hayley Bourn

doi:10.26182/4mr1-s727

Thermal Trauma and the Effect of Temperature and Time on the Morphology and DNA of Dentition

Hayley Bourn

School of Human Sciences

Research output: Thesis › Master's Thesis

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Abstract

This study analysed morphology and DNA degradation of dentition exposed to thermal trauma. Porcine dentition was subjected to forensically relevant thermal conditions of 500 to 900°C for 10 to 30 minutes, after which morphology, and DNA quality/ quantity was analysed. Increased thermal conditions increased morphological alterations and decreased DNA quantity/purity.
Significant DNA degradation, affecting subsequent downstream DNA application, was observed after 500°C for 15 minutes. This project expands upon existing research to assist investigators in establishing (visually) the likelihood of DNA recovery, thereby allowing the determination as to how worthwhile it is to perform subsequent time consuming and expensive molecular analyses.

Original language	English
Qualification	Masters
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Gaudieri, Silvana, Supervisor Franklin, Daniel, Supervisor Flavel, Ambika, Supervisor
Award date	19 Oct 2020
DOIs	https://doi.org/10.26182/4mr1-s727
Publication status	Unpublished - 2020

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10.26182/4mr1-s727

THESIS - MASTER OF FORENSIC SCIENCE - BOURN, Hayley Sue - 2020
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Cite this

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AB - This study analysed morphology and DNA degradation of dentition exposed to thermal trauma. Porcine dentition was subjected to forensically relevant thermal conditions of 500 to 900°C for 10 to 30 minutes, after which morphology, and DNA quality/ quantity was analysed. Increased thermal conditions increased morphological alterations and decreased DNA quantity/purity. Significant DNA degradation, affecting subsequent downstream DNA application, was observed after 500°C for 15 minutes. This project expands upon existing research to assist investigators in establishing (visually) the likelihood of DNA recovery, thereby allowing the determination as to how worthwhile it is to perform subsequent time consuming and expensive molecular analyses.

KW - thermal trauma

KW - forensic science

KW - DNA analyses

KW - burned bone

KW - bone morphology

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Thermal Trauma and the Effect of Temperature and Time on the Morphology and DNA of Dentition

Abstract

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