Thermal Trauma and the Effect of Temperature and Time on the Morphology and DNA of Dentition

Hayley Bourn

Research output: ThesisDoctoral Thesis

13 Downloads (Pure)

Abstract

This study analysed morphology and DNA degradation of dentition exposed to thermal trauma. Porcine dentition was subjected to forensically relevant thermal conditions of 500 to 900°C for 10 to 30 minutes, after which morphology, and DNA quality/ quantity was analysed. Increased thermal conditions increased morphological alterations and decreased DNA quantity/purity.
Significant DNA degradation, affecting subsequent downstream DNA application, was observed after 500°C for 15 minutes. This project expands upon existing research to assist investigators in establishing (visually) the likelihood of DNA recovery, thereby allowing the determination as to how worthwhile it is to perform subsequent time consuming and expensive molecular analyses.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Gaudieri, Silvana, Supervisor
  • Franklin, Daniel, Supervisor
  • Flavel, Ambika, Supervisor
Award date19 Oct 2020
DOIs
Publication statusUnpublished - 2020

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