The use of monoclonal antibody Y1/82A in the identification of acute myeloblastic and monocytic leukemias

F. R. Davey, W. N. Erber, K. C. Gatter, D. Y. Mason

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A newly developed monoclonal antibody (Y1/82A), which binds a cytoplasmic antigen in peripheral blood monocytes and tissue macrophages, was tested for its ability to detect monocytic differentiation in acute myeloid leukemia, i.e., to distinguish French-American-British (FAB) groups M4 and M5 from FAB groups M1, M2, M3, M6, and M7. Staining was performed by the alkaline phosphatase-antialkaline phosphatase (APAAP) immunocytochemical technic on bone marrow smears from 29 cases of acute myeloid leukemia, on 17 normal peripheral blood and/or bone marrow smears, on bone marrows from 10 cases of lymphoid leukemia, and on lymph nodes of 13 patients with lymphoma. Neoplastic cells from 11 of 11 patients with either M4 or M5 leukemia had positive results, whereas only 2 out of 18 cases of M1, M2, M3, M6, and M7 leukemia had positive results. In normal samples, only peripheral blood monocytes, bone marrow macrophages, and megakaryocytes stained. Lymphoid neoplasms were unreactive. These results suggest that monoclonal antibody Y1/82A may be a useful reagent in detecting cases of M4 and M5 acute myeloid leukemia and that it offers a valuable alternative to nonspecific esterase cytochemistry.

Original languageEnglish
Pages (from-to)76-80
Number of pages5
JournalAmerican Journal of Clinical Pathology
Volume89
Issue number1
DOIs
Publication statusPublished - 1 Jan 1988
Externally publishedYes

Fingerprint

Leukemia, Monocytic, Acute
Acute Myeloid Leukemia
Bone Marrow
Monoclonal Antibodies
Monocytes
Leukemia
Macrophages
Leukemia, Myelomonocytic, Acute
Lymphoid Leukemia
Histocytochemistry
Carboxylesterase
Megakaryocytes
Phosphoric Monoester Hydrolases
Alkaline Phosphatase
Lymphoma
Lymph Nodes
Staining and Labeling
Antigens
Neoplasms

Cite this

@article{40fe904cf7554163bfcf44bb8d952b17,
title = "The use of monoclonal antibody Y1/82A in the identification of acute myeloblastic and monocytic leukemias",
abstract = "A newly developed monoclonal antibody (Y1/82A), which binds a cytoplasmic antigen in peripheral blood monocytes and tissue macrophages, was tested for its ability to detect monocytic differentiation in acute myeloid leukemia, i.e., to distinguish French-American-British (FAB) groups M4 and M5 from FAB groups M1, M2, M3, M6, and M7. Staining was performed by the alkaline phosphatase-antialkaline phosphatase (APAAP) immunocytochemical technic on bone marrow smears from 29 cases of acute myeloid leukemia, on 17 normal peripheral blood and/or bone marrow smears, on bone marrows from 10 cases of lymphoid leukemia, and on lymph nodes of 13 patients with lymphoma. Neoplastic cells from 11 of 11 patients with either M4 or M5 leukemia had positive results, whereas only 2 out of 18 cases of M1, M2, M3, M6, and M7 leukemia had positive results. In normal samples, only peripheral blood monocytes, bone marrow macrophages, and megakaryocytes stained. Lymphoid neoplasms were unreactive. These results suggest that monoclonal antibody Y1/82A may be a useful reagent in detecting cases of M4 and M5 acute myeloid leukemia and that it offers a valuable alternative to nonspecific esterase cytochemistry.",
author = "Davey, {F. R.} and Erber, {W. N.} and Gatter, {K. C.} and Mason, {D. Y.}",
year = "1988",
month = "1",
day = "1",
doi = "10.1093/ajcp/89.1.76",
language = "English",
volume = "89",
pages = "76--80",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "1",

}

The use of monoclonal antibody Y1/82A in the identification of acute myeloblastic and monocytic leukemias. / Davey, F. R.; Erber, W. N.; Gatter, K. C.; Mason, D. Y.

In: American Journal of Clinical Pathology, Vol. 89, No. 1, 01.01.1988, p. 76-80.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The use of monoclonal antibody Y1/82A in the identification of acute myeloblastic and monocytic leukemias

AU - Davey, F. R.

AU - Erber, W. N.

AU - Gatter, K. C.

AU - Mason, D. Y.

PY - 1988/1/1

Y1 - 1988/1/1

N2 - A newly developed monoclonal antibody (Y1/82A), which binds a cytoplasmic antigen in peripheral blood monocytes and tissue macrophages, was tested for its ability to detect monocytic differentiation in acute myeloid leukemia, i.e., to distinguish French-American-British (FAB) groups M4 and M5 from FAB groups M1, M2, M3, M6, and M7. Staining was performed by the alkaline phosphatase-antialkaline phosphatase (APAAP) immunocytochemical technic on bone marrow smears from 29 cases of acute myeloid leukemia, on 17 normal peripheral blood and/or bone marrow smears, on bone marrows from 10 cases of lymphoid leukemia, and on lymph nodes of 13 patients with lymphoma. Neoplastic cells from 11 of 11 patients with either M4 or M5 leukemia had positive results, whereas only 2 out of 18 cases of M1, M2, M3, M6, and M7 leukemia had positive results. In normal samples, only peripheral blood monocytes, bone marrow macrophages, and megakaryocytes stained. Lymphoid neoplasms were unreactive. These results suggest that monoclonal antibody Y1/82A may be a useful reagent in detecting cases of M4 and M5 acute myeloid leukemia and that it offers a valuable alternative to nonspecific esterase cytochemistry.

AB - A newly developed monoclonal antibody (Y1/82A), which binds a cytoplasmic antigen in peripheral blood monocytes and tissue macrophages, was tested for its ability to detect monocytic differentiation in acute myeloid leukemia, i.e., to distinguish French-American-British (FAB) groups M4 and M5 from FAB groups M1, M2, M3, M6, and M7. Staining was performed by the alkaline phosphatase-antialkaline phosphatase (APAAP) immunocytochemical technic on bone marrow smears from 29 cases of acute myeloid leukemia, on 17 normal peripheral blood and/or bone marrow smears, on bone marrows from 10 cases of lymphoid leukemia, and on lymph nodes of 13 patients with lymphoma. Neoplastic cells from 11 of 11 patients with either M4 or M5 leukemia had positive results, whereas only 2 out of 18 cases of M1, M2, M3, M6, and M7 leukemia had positive results. In normal samples, only peripheral blood monocytes, bone marrow macrophages, and megakaryocytes stained. Lymphoid neoplasms were unreactive. These results suggest that monoclonal antibody Y1/82A may be a useful reagent in detecting cases of M4 and M5 acute myeloid leukemia and that it offers a valuable alternative to nonspecific esterase cytochemistry.

UR - http://www.scopus.com/inward/record.url?scp=0023873867&partnerID=8YFLogxK

U2 - 10.1093/ajcp/89.1.76

DO - 10.1093/ajcp/89.1.76

M3 - Article

VL - 89

SP - 76

EP - 80

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 1

ER -