The transcriptome of sesquiterpenoid biosynthesis in heartwood xylem of Western Australian sandalwood (Santalum spicatum)

J. Moniodis, Chris Jones, Liz Barbour, Julie Plummer, Emilio Ghisalberti, J. Bohlmann

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

© 2014 Elsevier Ltd. All rights reserved. The fragrant heartwood oil of West Australian sandalwood (Santalum spicatum) contains a mixture of sesquiterpene olefins and alcohols, including variable levels of the valuable sesquiterpene alcohols, α- and β-santalol, and often high levels of E,E-farnesol. Transcriptome analysis revealed sequences for a nearly complete set of genes of the sesquiterpenoid biosynthetic pathway in this commercially valuable sandalwood species. Transcriptome sequences were produced from heartwood xylem tissue of a farnesol-rich individual tree. From the assembly of 12,537 contigs, seven different terpene synthases (TPSs), several cytochromes P450, and allylic phosphatases were identified, as well as transcripts of the mevalonic acid and methylerythritol phosphate pathways. Five of the S. spicatum TPS sequences were previously unknown. The full-length cDNA of SspiTPS4 was cloned and the enzyme functionally characterized as a multi-product sesquisabinene B synthase, which complements previous characterization of santalene and bisabolol synthases in S. spicatum. While SspiTPS4 and previously cloned sandalwood TPSs do not explain the prevalence of E,E-farnesol in S. spicatum, the genes identified in this and previous work can form a basis for future studies on natural variation of sandalwood terpenoid oil profiles.
Original languageEnglish
Pages (from-to)79-86
JournalPhytochemistry
Volume113
DOIs
Publication statusPublished - 2015

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Santalum
Santalum spicatum
Farnesol
Xylem
Biosynthesis
heartwood
farnesol
Transcriptome
transcriptome
terpenoids
sesquiterpenoids
xylem
Sesquiterpenes
biosynthesis
Oils
Genes
Alcohols
Mevalonic Acid
alcohols
Terpenes

Cite this

Moniodis, J. ; Jones, Chris ; Barbour, Liz ; Plummer, Julie ; Ghisalberti, Emilio ; Bohlmann, J. / The transcriptome of sesquiterpenoid biosynthesis in heartwood xylem of Western Australian sandalwood (Santalum spicatum). In: Phytochemistry. 2015 ; Vol. 113. pp. 79-86.
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The transcriptome of sesquiterpenoid biosynthesis in heartwood xylem of Western Australian sandalwood (Santalum spicatum). / Moniodis, J.; Jones, Chris; Barbour, Liz; Plummer, Julie; Ghisalberti, Emilio; Bohlmann, J.

In: Phytochemistry, Vol. 113, 2015, p. 79-86.

Research output: Contribution to journalArticle

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AB - © 2014 Elsevier Ltd. All rights reserved. The fragrant heartwood oil of West Australian sandalwood (Santalum spicatum) contains a mixture of sesquiterpene olefins and alcohols, including variable levels of the valuable sesquiterpene alcohols, α- and β-santalol, and often high levels of E,E-farnesol. Transcriptome analysis revealed sequences for a nearly complete set of genes of the sesquiterpenoid biosynthetic pathway in this commercially valuable sandalwood species. Transcriptome sequences were produced from heartwood xylem tissue of a farnesol-rich individual tree. From the assembly of 12,537 contigs, seven different terpene synthases (TPSs), several cytochromes P450, and allylic phosphatases were identified, as well as transcripts of the mevalonic acid and methylerythritol phosphate pathways. Five of the S. spicatum TPS sequences were previously unknown. The full-length cDNA of SspiTPS4 was cloned and the enzyme functionally characterized as a multi-product sesquisabinene B synthase, which complements previous characterization of santalene and bisabolol synthases in S. spicatum. While SspiTPS4 and previously cloned sandalwood TPSs do not explain the prevalence of E,E-farnesol in S. spicatum, the genes identified in this and previous work can form a basis for future studies on natural variation of sandalwood terpenoid oil profiles.

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