TY - JOUR
T1 - The thyroid stimulating hormone receptor in human disease
AU - Harrison, L. C.
AU - Leedman, P. J.
PY - 1990/2
Y1 - 1990/2
N2 - The initial step in the action of thyrotropin (TSH) is its binding to the TSH receptor. TSH receptor antibodies are detected in up to 90% of patients with Graves' disease. Serial measurements of TSH receptor antibodies in patients with Graves' hyperthyroidism are helpful in predicting relapse. The TSH receptor was purified using affinity chromatography on wheat germ lectin agarose and TSH-agarose. Using an immunoblotting technique to characterize the TSH receptor, it was found to be an oligomeric glycoprotein consisting of three noncovalently bound subunits of Mr ∼ 70,000, ∼ 50,000 and ∼ 35,000 which on reduction yield a single subunit of Mr ∼ 25,000.
AB - The initial step in the action of thyrotropin (TSH) is its binding to the TSH receptor. TSH receptor antibodies are detected in up to 90% of patients with Graves' disease. Serial measurements of TSH receptor antibodies in patients with Graves' hyperthyroidism are helpful in predicting relapse. The TSH receptor was purified using affinity chromatography on wheat germ lectin agarose and TSH-agarose. Using an immunoblotting technique to characterize the TSH receptor, it was found to be an oligomeric glycoprotein consisting of three noncovalently bound subunits of Mr ∼ 70,000, ∼ 50,000 and ∼ 35,000 which on reduction yield a single subunit of Mr ∼ 25,000.
KW - thyrotropin
KW - TSH receptor
UR - http://www.scopus.com/inward/record.url?scp=0025237059&partnerID=8YFLogxK
U2 - 10.1016/0009-9120(90)90417-S
DO - 10.1016/0009-9120(90)90417-S
M3 - Article
C2 - 1691960
AN - SCOPUS:0025237059
SN - 0009-9120
VL - 23
SP - 43
EP - 48
JO - Clinical Biochemistry
JF - Clinical Biochemistry
IS - 1
ER -
