The role of jasmonate signalling in quinolizidine alkaloid biosynthesis, wounding and aphid predation response in narrow-leafed lupin

Karen M. Frick, Rhonda C. Foley, Kadambot H. M. Siddique, Karam B. Singh, Lars G. Kamphuis

Research output: Contribution to journalArticle

Abstract

Quinolizidine alkaloids (QAs) are toxic secondary metabolites produced in lupin species that protect the plant against insects. They form in vegetative tissues and accumulate to a different extent in the grains: high levels in 'bitter' narrow-leafed lupin (NLL) and low levels in 'sweet' NLL. Grain QA levels vary considerably, and sometimes exceed the industry limit for food and feed purposes. We hypothesised that jasmonates regulate QA biosynthesis in response to environmental stresses such as wounding and aphid predation, which may explain non-genetic variability in grain QA levels. Methyl jasmonate (MeJA)-inducible genes were identified and verified in NLL. Exogenous MeJA application-induced expression of QA biosynthetic genes and QA levels for bitter, but not sweet NLL. Although MeJA-inducible genes responded to wounding, the expression of QA biosynthetic genes was not induced for bitter and sweet NLL. We assessed the effect of aphid predation on QA production for two cultivars - one moderately resistant and one susceptible to aphid predation. Although MeJA-inducible genes responded to aphid predation, no change in QA levels was found for either cultivar. These findings offer insights into the regulation of QA biosynthesis in bitter and sweet NLL and concludes that aphids are not a concern for increasing grain QAs in NLL cultivars.

Original languageEnglish
Pages (from-to)443-454
Number of pages12
JournalFunctional Plant Biology
Volume46
Issue number5
DOIs
Publication statusPublished - 2019

Cite this

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title = "The role of jasmonate signalling in quinolizidine alkaloid biosynthesis, wounding and aphid predation response in narrow-leafed lupin",
abstract = "Quinolizidine alkaloids (QAs) are toxic secondary metabolites produced in lupin species that protect the plant against insects. They form in vegetative tissues and accumulate to a different extent in the grains: high levels in 'bitter' narrow-leafed lupin (NLL) and low levels in 'sweet' NLL. Grain QA levels vary considerably, and sometimes exceed the industry limit for food and feed purposes. We hypothesised that jasmonates regulate QA biosynthesis in response to environmental stresses such as wounding and aphid predation, which may explain non-genetic variability in grain QA levels. Methyl jasmonate (MeJA)-inducible genes were identified and verified in NLL. Exogenous MeJA application-induced expression of QA biosynthetic genes and QA levels for bitter, but not sweet NLL. Although MeJA-inducible genes responded to wounding, the expression of QA biosynthetic genes was not induced for bitter and sweet NLL. We assessed the effect of aphid predation on QA production for two cultivars - one moderately resistant and one susceptible to aphid predation. Although MeJA-inducible genes responded to aphid predation, no change in QA levels was found for either cultivar. These findings offer insights into the regulation of QA biosynthesis in bitter and sweet NLL and concludes that aphids are not a concern for increasing grain QAs in NLL cultivars.",
keywords = "biotic stress, grain quality, green-peach aphid, Myzus persicae, plant defence, plant defense, secondary metabolism, PLANT STRESS-RESPONSE, GENE-EXPRESSION, NICOTIANA-ATTENUATA, ANGUSTIFOLIUS, ACCUMULATION, RESISTANCE, TRANSDUCTION, INDUCTION, PROFILES, GENOTYPE",
author = "Frick, {Karen M.} and Foley, {Rhonda C.} and Siddique, {Kadambot H. M.} and Singh, {Karam B.} and Kamphuis, {Lars G.}",
year = "2019",
doi = "10.1071/FP18278",
language = "English",
volume = "46",
pages = "443--454",
journal = "Functional Plant Biology",
issn = "1445-4408",
publisher = "CSIRO Publishing",
number = "5",

}

TY - JOUR

T1 - The role of jasmonate signalling in quinolizidine alkaloid biosynthesis, wounding and aphid predation response in narrow-leafed lupin

AU - Frick, Karen M.

AU - Foley, Rhonda C.

AU - Siddique, Kadambot H. M.

AU - Singh, Karam B.

AU - Kamphuis, Lars G.

PY - 2019

Y1 - 2019

N2 - Quinolizidine alkaloids (QAs) are toxic secondary metabolites produced in lupin species that protect the plant against insects. They form in vegetative tissues and accumulate to a different extent in the grains: high levels in 'bitter' narrow-leafed lupin (NLL) and low levels in 'sweet' NLL. Grain QA levels vary considerably, and sometimes exceed the industry limit for food and feed purposes. We hypothesised that jasmonates regulate QA biosynthesis in response to environmental stresses such as wounding and aphid predation, which may explain non-genetic variability in grain QA levels. Methyl jasmonate (MeJA)-inducible genes were identified and verified in NLL. Exogenous MeJA application-induced expression of QA biosynthetic genes and QA levels for bitter, but not sweet NLL. Although MeJA-inducible genes responded to wounding, the expression of QA biosynthetic genes was not induced for bitter and sweet NLL. We assessed the effect of aphid predation on QA production for two cultivars - one moderately resistant and one susceptible to aphid predation. Although MeJA-inducible genes responded to aphid predation, no change in QA levels was found for either cultivar. These findings offer insights into the regulation of QA biosynthesis in bitter and sweet NLL and concludes that aphids are not a concern for increasing grain QAs in NLL cultivars.

AB - Quinolizidine alkaloids (QAs) are toxic secondary metabolites produced in lupin species that protect the plant against insects. They form in vegetative tissues and accumulate to a different extent in the grains: high levels in 'bitter' narrow-leafed lupin (NLL) and low levels in 'sweet' NLL. Grain QA levels vary considerably, and sometimes exceed the industry limit for food and feed purposes. We hypothesised that jasmonates regulate QA biosynthesis in response to environmental stresses such as wounding and aphid predation, which may explain non-genetic variability in grain QA levels. Methyl jasmonate (MeJA)-inducible genes were identified and verified in NLL. Exogenous MeJA application-induced expression of QA biosynthetic genes and QA levels for bitter, but not sweet NLL. Although MeJA-inducible genes responded to wounding, the expression of QA biosynthetic genes was not induced for bitter and sweet NLL. We assessed the effect of aphid predation on QA production for two cultivars - one moderately resistant and one susceptible to aphid predation. Although MeJA-inducible genes responded to aphid predation, no change in QA levels was found for either cultivar. These findings offer insights into the regulation of QA biosynthesis in bitter and sweet NLL and concludes that aphids are not a concern for increasing grain QAs in NLL cultivars.

KW - biotic stress

KW - grain quality

KW - green-peach aphid

KW - Myzus persicae

KW - plant defence

KW - plant defense

KW - secondary metabolism

KW - PLANT STRESS-RESPONSE

KW - GENE-EXPRESSION

KW - NICOTIANA-ATTENUATA

KW - ANGUSTIFOLIUS

KW - ACCUMULATION

KW - RESISTANCE

KW - TRANSDUCTION

KW - INDUCTION

KW - PROFILES

KW - GENOTYPE

U2 - 10.1071/FP18278

DO - 10.1071/FP18278

M3 - Article

VL - 46

SP - 443

EP - 454

JO - Functional Plant Biology

JF - Functional Plant Biology

SN - 1445-4408

IS - 5

ER -