The Role of Insulin-Like Growth Factor-I (IGF-I) and Estradiol in Rabbit Corpus Luteum Progesterone Production

S.H. Chen; V. Zanagnolo; S. Preutthipan; K.P. Roberts; S.B. Goodman; Arunasalam Dharmarajan

doi:10.1007/BF02738805

The Role of Insulin-Like Growth Factor-I (IGF-I) and Estradiol in Rabbit Corpus Luteum Progesterone Production

S.H. Chen, V. Zanagnolo, S. Preutthipan, K.P. Roberts, S.B. Goodman, Arunasalam Dharmarajan

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Abstract

To determine whether insulin-like growth factor-I (IGF-I) plays a role in rabbit corpus luteum (CL) physiology the authors examined: IGF-I expression, the effect of IGF-I on progesterone (P) production in vitro, and the interaction of IGF-I with estradiol, the primary luteotropin in the rabbit. Northern blot analysis revealed that IGF-I mRNA is present in the rabbit CL throughout pseudopregnancy. An intact ovarian in vitro perfusion model and dispersed luteal cell culture were used to determine the effects of IGF-I on P production and interactions with estradiol. IGF-I significantly stimulated P production compared to control medium by the isolated, intact perfused rabbit ovary and by dispersed, cultured luteal cells. Estradiol alone did not stimulate P production in vitro. Estradiol did augment IGF-I-stimulated P production in the intact perfused ovary and in luteal cell culture. These findings support a role for IGF-I in rabbit CL P production.

Original language	English
Pages (from-to)	73-77
Journal	Endocrine
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/BF02738805
Publication status	Published - 1997

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title = "The Role of Insulin-Like Growth Factor-I (IGF-I) and Estradiol in Rabbit Corpus Luteum Progesterone Production",

abstract = "To determine whether insulin-like growth factor-I (IGF-I) plays a role in rabbit corpus luteum (CL) physiology the authors examined: IGF-I expression, the effect of IGF-I on progesterone (P) production in vitro, and the interaction of IGF-I with estradiol, the primary luteotropin in the rabbit. Northern blot analysis revealed that IGF-I mRNA is present in the rabbit CL throughout pseudopregnancy. An intact ovarian in vitro perfusion model and dispersed luteal cell culture were used to determine the effects of IGF-I on P production and interactions with estradiol. IGF-I significantly stimulated P production compared to control medium by the isolated, intact perfused rabbit ovary and by dispersed, cultured luteal cells. Estradiol alone did not stimulate P production in vitro. Estradiol did augment IGF-I-stimulated P production in the intact perfused ovary and in luteal cell culture. These findings support a role for IGF-I in rabbit CL P production.",

author = "S.H. Chen and V. Zanagnolo and S. Preutthipan and K.P. Roberts and S.B. Goodman and Arunasalam Dharmarajan",

year = "1997",

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T1 - The Role of Insulin-Like Growth Factor-I (IGF-I) and Estradiol in Rabbit Corpus Luteum Progesterone Production

AU - Chen, S.H.

AU - Zanagnolo, V.

AU - Preutthipan, S.

AU - Roberts, K.P.

AU - Goodman, S.B.

AU - Dharmarajan, Arunasalam

PY - 1997

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AB - To determine whether insulin-like growth factor-I (IGF-I) plays a role in rabbit corpus luteum (CL) physiology the authors examined: IGF-I expression, the effect of IGF-I on progesterone (P) production in vitro, and the interaction of IGF-I with estradiol, the primary luteotropin in the rabbit. Northern blot analysis revealed that IGF-I mRNA is present in the rabbit CL throughout pseudopregnancy. An intact ovarian in vitro perfusion model and dispersed luteal cell culture were used to determine the effects of IGF-I on P production and interactions with estradiol. IGF-I significantly stimulated P production compared to control medium by the isolated, intact perfused rabbit ovary and by dispersed, cultured luteal cells. Estradiol alone did not stimulate P production in vitro. Estradiol did augment IGF-I-stimulated P production in the intact perfused ovary and in luteal cell culture. These findings support a role for IGF-I in rabbit CL P production.

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DO - 10.1007/BF02738805

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JO - Endocrine

JF - Endocrine

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The Role of Insulin-Like Growth Factor-I (IGF-I) and Estradiol in Rabbit Corpus Luteum Progesterone Production

Abstract

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