The role of chronic infection in children with otitis media with effusion: Evidence for intracellular persistence of bacteria

Harvey Coates, R. Thornton, J. Langlands, P. Filion, A.D. Keil, Shyan Vijayasekaran, Peter Richmond

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    Abstract

    OBJECTIVE: Demonstrate mucosal bacterial infection in children with otitis media with effusion (OME).STUDY DESIGN AND SETTING: Middle ear mucosal biopsies from 11 children with OME were examined for bacteria utilizing transmission electron microscopy. This was correlated with standard culture and polymerase chain reaction (PCR) of middle ear effusions.RESULTS: Gram-positive coccal bacteria were demonstrated in middle ear mucosal epithelial cells of 4 of 11 (36%) children. Morphological appearance of bacteria and detection of pneumolysin DNA by PCR in middle ear fluid suggests a role for persistent intracellular infection with Streptococcus pneumoniae and other gram-positive cocci in some cases of OME.CONCLUSION: Intracellular bacterial infection of middle ear mucosal epithelial cells in children with OME may be an important mechanism for bacterial persistence, and contribute to inflammation and mucus production in the pathogenesis of this condition.SIGNIFICANCE: Persistent intracellular infection is a novel paradigm for OME pathogenesis in children and may influence antibiotic effectiveness in treatment of this condition. (C) 2008 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.
    Original languageEnglish
    Pages (from-to)778-781
    JournalAmerican Academy of Otolaryngology - Head and Neck Surgery, Bulletin
    Volume138
    Issue number6
    DOIs
    Publication statusPublished - 2008

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    Otitis Media with Effusion
    Middle Ear
    Bacteria
    Infection
    Bacterial Infections
    Epithelial Cells
    Pneumococcal Infections
    Gram-Positive Cocci
    Polymerase Chain Reaction
    Gram-Positive Bacteria
    DNA-Directed DNA Polymerase
    Mucus
    Transmission Electron Microscopy
    Anti-Bacterial Agents
    Inflammation
    Biopsy

    Cite this

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    title = "The role of chronic infection in children with otitis media with effusion: Evidence for intracellular persistence of bacteria",
    abstract = "OBJECTIVE: Demonstrate mucosal bacterial infection in children with otitis media with effusion (OME).STUDY DESIGN AND SETTING: Middle ear mucosal biopsies from 11 children with OME were examined for bacteria utilizing transmission electron microscopy. This was correlated with standard culture and polymerase chain reaction (PCR) of middle ear effusions.RESULTS: Gram-positive coccal bacteria were demonstrated in middle ear mucosal epithelial cells of 4 of 11 (36{\%}) children. Morphological appearance of bacteria and detection of pneumolysin DNA by PCR in middle ear fluid suggests a role for persistent intracellular infection with Streptococcus pneumoniae and other gram-positive cocci in some cases of OME.CONCLUSION: Intracellular bacterial infection of middle ear mucosal epithelial cells in children with OME may be an important mechanism for bacterial persistence, and contribute to inflammation and mucus production in the pathogenesis of this condition.SIGNIFICANCE: Persistent intracellular infection is a novel paradigm for OME pathogenesis in children and may influence antibiotic effectiveness in treatment of this condition. (C) 2008 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.",
    author = "Harvey Coates and R. Thornton and J. Langlands and P. Filion and A.D. Keil and Shyan Vijayasekaran and Peter Richmond",
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    language = "English",
    volume = "138",
    pages = "778--781",
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    TY - JOUR

    T1 - The role of chronic infection in children with otitis media with effusion: Evidence for intracellular persistence of bacteria

    AU - Coates, Harvey

    AU - Thornton, R.

    AU - Langlands, J.

    AU - Filion, P.

    AU - Keil, A.D.

    AU - Vijayasekaran, Shyan

    AU - Richmond, Peter

    PY - 2008

    Y1 - 2008

    N2 - OBJECTIVE: Demonstrate mucosal bacterial infection in children with otitis media with effusion (OME).STUDY DESIGN AND SETTING: Middle ear mucosal biopsies from 11 children with OME were examined for bacteria utilizing transmission electron microscopy. This was correlated with standard culture and polymerase chain reaction (PCR) of middle ear effusions.RESULTS: Gram-positive coccal bacteria were demonstrated in middle ear mucosal epithelial cells of 4 of 11 (36%) children. Morphological appearance of bacteria and detection of pneumolysin DNA by PCR in middle ear fluid suggests a role for persistent intracellular infection with Streptococcus pneumoniae and other gram-positive cocci in some cases of OME.CONCLUSION: Intracellular bacterial infection of middle ear mucosal epithelial cells in children with OME may be an important mechanism for bacterial persistence, and contribute to inflammation and mucus production in the pathogenesis of this condition.SIGNIFICANCE: Persistent intracellular infection is a novel paradigm for OME pathogenesis in children and may influence antibiotic effectiveness in treatment of this condition. (C) 2008 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.

    AB - OBJECTIVE: Demonstrate mucosal bacterial infection in children with otitis media with effusion (OME).STUDY DESIGN AND SETTING: Middle ear mucosal biopsies from 11 children with OME were examined for bacteria utilizing transmission electron microscopy. This was correlated with standard culture and polymerase chain reaction (PCR) of middle ear effusions.RESULTS: Gram-positive coccal bacteria were demonstrated in middle ear mucosal epithelial cells of 4 of 11 (36%) children. Morphological appearance of bacteria and detection of pneumolysin DNA by PCR in middle ear fluid suggests a role for persistent intracellular infection with Streptococcus pneumoniae and other gram-positive cocci in some cases of OME.CONCLUSION: Intracellular bacterial infection of middle ear mucosal epithelial cells in children with OME may be an important mechanism for bacterial persistence, and contribute to inflammation and mucus production in the pathogenesis of this condition.SIGNIFICANCE: Persistent intracellular infection is a novel paradigm for OME pathogenesis in children and may influence antibiotic effectiveness in treatment of this condition. (C) 2008 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.

    U2 - 10.1016/j.otohns.2007.02.009

    DO - 10.1016/j.otohns.2007.02.009

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    SP - 778

    EP - 781

    JO - American Academy of Otolaryngology - Head and Neck Surgery, Bulletin

    JF - American Academy of Otolaryngology - Head and Neck Surgery, Bulletin

    IS - 6

    ER -