The rice mitochondria proteome and its response during development and to the environment

Shaobai Huang, Rachel Shingaki-Wells, Nicolas Taylor, Harvey Millar

Research output: Contribution to journalReview article

23 Citations (Scopus)

Abstract

Rice (Oryza sativa L.) is both a major crop species and the key model grass for molecular and physiological research. Mitochondria are important in rice, as in all crops, as the main source of ATP for cell maintenance and growth. However, the practical significance of understanding the function of mitochondria in rice is increased by the widespread farming practice of using hybrids to boost rice production. This relies on cytoplasmic male sterile (CMS) lines with abortive pollen caused by dysfunctional mitochondria. We provide an overview of what is known about the mitochondrial proteome of rice seedlings. To date, more than 320 proteins have been identified in purified rice mitochondria using mass spectrometry. The insights from this work include a broad understanding of the major subunits of mitochondrial respiratory complexes and TCA cycle enzymes, carbon and nitrogen metabolism enzymes as well as details of the supporting machinery for biogenesis and the subset of stress-responsive mitochondrial proteins. Many proteins with unknown functions have also been found in rice mitochondria. Proteomic analysis has also revealed the features of rice mitochondrial protein presequences required for mitochondrial targeting, as well as cleavage site features for processing of precursors after import. Changes in the abundance of rice mitochondrial proteins in response to different stresses, especially anoxia and light, are summarized. Future research on quantitative analysis of the rice mitochondrial proteomes at the spatial and developmental level, its response to environmental stresses and recent advances in understanding of the basis of rice CMS systems are highlighted. © 2013 Huang, Shingaki-Wells, Taylor and Millar.
Original languageEnglish
Pages (from-to)16
JournalFrontiers in Plant Science
Volume4
Issue number16
DOIs
Publication statusPublished - Feb 2013

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proteome
mitochondria
rice
proteins
nitrogen metabolism
crops
enzymes
proteomics
imports
quantitative analysis
hypoxia
stress response
Oryza sativa
farming systems
mass spectrometry
pollen
grasses
seedlings
carbon

Cite this

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title = "The rice mitochondria proteome and its response during development and to the environment",
abstract = "Rice (Oryza sativa L.) is both a major crop species and the key model grass for molecular and physiological research. Mitochondria are important in rice, as in all crops, as the main source of ATP for cell maintenance and growth. However, the practical significance of understanding the function of mitochondria in rice is increased by the widespread farming practice of using hybrids to boost rice production. This relies on cytoplasmic male sterile (CMS) lines with abortive pollen caused by dysfunctional mitochondria. We provide an overview of what is known about the mitochondrial proteome of rice seedlings. To date, more than 320 proteins have been identified in purified rice mitochondria using mass spectrometry. The insights from this work include a broad understanding of the major subunits of mitochondrial respiratory complexes and TCA cycle enzymes, carbon and nitrogen metabolism enzymes as well as details of the supporting machinery for biogenesis and the subset of stress-responsive mitochondrial proteins. Many proteins with unknown functions have also been found in rice mitochondria. Proteomic analysis has also revealed the features of rice mitochondrial protein presequences required for mitochondrial targeting, as well as cleavage site features for processing of precursors after import. Changes in the abundance of rice mitochondrial proteins in response to different stresses, especially anoxia and light, are summarized. Future research on quantitative analysis of the rice mitochondrial proteomes at the spatial and developmental level, its response to environmental stresses and recent advances in understanding of the basis of rice CMS systems are highlighted. {\circledC} 2013 Huang, Shingaki-Wells, Taylor and Millar.",
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The rice mitochondria proteome and its response during development and to the environment. / Huang, Shaobai; Shingaki-Wells, Rachel; Taylor, Nicolas; Millar, Harvey.

In: Frontiers in Plant Science, Vol. 4, No. 16, 02.2013, p. 16.

Research output: Contribution to journalReview article

TY - JOUR

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AU - Taylor, Nicolas

AU - Millar, Harvey

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AB - Rice (Oryza sativa L.) is both a major crop species and the key model grass for molecular and physiological research. Mitochondria are important in rice, as in all crops, as the main source of ATP for cell maintenance and growth. However, the practical significance of understanding the function of mitochondria in rice is increased by the widespread farming practice of using hybrids to boost rice production. This relies on cytoplasmic male sterile (CMS) lines with abortive pollen caused by dysfunctional mitochondria. We provide an overview of what is known about the mitochondrial proteome of rice seedlings. To date, more than 320 proteins have been identified in purified rice mitochondria using mass spectrometry. The insights from this work include a broad understanding of the major subunits of mitochondrial respiratory complexes and TCA cycle enzymes, carbon and nitrogen metabolism enzymes as well as details of the supporting machinery for biogenesis and the subset of stress-responsive mitochondrial proteins. Many proteins with unknown functions have also been found in rice mitochondria. Proteomic analysis has also revealed the features of rice mitochondrial protein presequences required for mitochondrial targeting, as well as cleavage site features for processing of precursors after import. Changes in the abundance of rice mitochondrial proteins in response to different stresses, especially anoxia and light, are summarized. Future research on quantitative analysis of the rice mitochondrial proteomes at the spatial and developmental level, its response to environmental stresses and recent advances in understanding of the basis of rice CMS systems are highlighted. © 2013 Huang, Shingaki-Wells, Taylor and Millar.

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