TY - JOUR
T1 - The rapid detection of cytotoxicity using a modified human sperm survival assay
AU - Iemmolo, M.
AU - Simmons, Leigh
AU - Matson, P.
PY - 2005
Y1 - 2005
N2 - Sperm were cultured at room temperature in seminal plasma, in the presence or absence of a test item or solution. Progressive motility of the sperm was measured using a Sperm Quality Analyser IIB. A progressive decline in motility of sperm was seen under control conditions from 0 hr to 24 hrs. Culture medium did not cause a significant decline in sperm survival index during the first 6 hrs of culture but did after 24 hrs. A dose dependent reduction in sperm survival index was then seen with formaldehyde. Powder-free rubber gloves proved extremely toxic unlike the associated rubber plunger of a non-pyrogenic syringe. It is concluded that the use of unwashed human sperm in survival studies allows detection of toxicity within 6 hrs, a time much less than conventional methods using washed human sperm.
AB - Sperm were cultured at room temperature in seminal plasma, in the presence or absence of a test item or solution. Progressive motility of the sperm was measured using a Sperm Quality Analyser IIB. A progressive decline in motility of sperm was seen under control conditions from 0 hr to 24 hrs. Culture medium did not cause a significant decline in sperm survival index during the first 6 hrs of culture but did after 24 hrs. A dose dependent reduction in sperm survival index was then seen with formaldehyde. Powder-free rubber gloves proved extremely toxic unlike the associated rubber plunger of a non-pyrogenic syringe. It is concluded that the use of unwashed human sperm in survival studies allows detection of toxicity within 6 hrs, a time much less than conventional methods using washed human sperm.
U2 - 10.1007/s10815-005-4916-4
DO - 10.1007/s10815-005-4916-4
M3 - Article
VL - 22
SP - 177
EP - 180
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
SN - 1058-0468
IS - 4
ER -