The persistence of biofilm-associated antibiotic resistance of Staphylococcus aureus isolated from clinical bovine mastitis cases in Australia

Charlene Babra, Jully G. Tiwari, Gerald Pier, Thi Ha Thein, Raju Sunagar, Srinivasaiah Sundareshan, Shrikrishna Isloor, Nagendra R. Hegde, Sharon de Wet, Margaret Deighton, Justine Gibson, Paul Costantino, John Wetherall, Trilochan Mukkur

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15 Citations (Scopus)

Abstract

The aim of this investigation was to determine the persistence of biofilm-associated antibiotic resistance developed by methicillin-sensitive Staphylococcus aureus (MSSA), of different capsular types, during biofilm formation. Because of superiority of the tissue culture plate (TCP) over the Congo Red Agar (CRA) method for measuring biofilm formation, it was used to determine the persistence of the antibiotic resistance developed by the isolates in biofilms. The antibiotic resistance was found to persist for 3-4 wk post-propagation as planktonic subcultures. Interestingly, some strains even developed resistance to vancomycin and/or teicoplanin. However, no association of either biofilm formation or persistent antibiotic resistance with the major capsular phenotype was observed. These observations highlight the potential significance of (a) determining the antibiograms of S. aureus subcultured from biofilms developed in vitro using the TCP method as well as from planktonic cultures for formulation of an optimal therapeutic strategy, and (b) continuing to identify predominant non-capsular antigens contributing to biofilm formation, regardless of the capsular phenotype for the development of an effective potentially broad-spectrum vaccine for prevention of bovine mastitis caused by S. aureus.

Original languageEnglish
Pages (from-to)469-474
Number of pages6
JournalFolia Microbiologica
Volume58
Issue number6
DOIs
Publication statusPublished - 1 Nov 2013
Externally publishedYes

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  • Cite this

    Babra, C., Tiwari, J. G., Pier, G., Thein, T. H., Sunagar, R., Sundareshan, S., Isloor, S., Hegde, N. R., de Wet, S., Deighton, M., Gibson, J., Costantino, P., Wetherall, J., & Mukkur, T. (2013). The persistence of biofilm-associated antibiotic resistance of Staphylococcus aureus isolated from clinical bovine mastitis cases in Australia. Folia Microbiologica, 58(6), 469-474. https://doi.org/10.1007/s12223-013-0232-z