The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds

Blake A. Miller, Aspinas Chapwanya, Tanya Kilminster, Tim Scanlon, John Milton, Hugo Osorio, Chris Oldham, Johan Greeff, Don R. Bergfelt, Alexandre M. O. Campos, Andre M. Almeida

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Seasonal weight loss (SWL) is a primary constraint for farmers in the Mediterranean and tropics. One cost-effective solution to SWL is utilizing breeds like the Damara sheep that have adapted to deal with nutritional stress. Previous studies concluded that one of the adaptation mechanisms of SWL is a specialized fatty acid metabolism. Accordingly, hepatic-mitochondrial proteomes were compared across two different breeds (24 sheep total, Merino, n = 12 and Damara, n = 12) and two different diets (restricted vs unrestricted diet, 6 per breed, per diet, 24 total). Mitochondrial-proteins were isolated and relatively quantified using Blue native PAGE/2D-electrophoresis and then analyzed via mass spectrometry. The tool ReviGO summarized the proteomes' gene-ontology terms. A total of 50 proteins were identified with 7 changing significantly in abundance (ANOVA p-value

Original languageEnglish
Article number0212580
Number of pages15
JournalPLoS One
Volume14
Issue number2
DOIs
Publication statusPublished - 20 Feb 2019

Cite this

Miller, B. A., Chapwanya, A., Kilminster, T., Scanlon, T., Milton, J., Osorio, H., ... Almeida, A. M. (2019). The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds. PLoS One, 14(2), [0212580]. https://doi.org/10.1371/journal.pone.0212580
Miller, Blake A. ; Chapwanya, Aspinas ; Kilminster, Tanya ; Scanlon, Tim ; Milton, John ; Osorio, Hugo ; Oldham, Chris ; Greeff, Johan ; Bergfelt, Don R. ; Campos, Alexandre M. O. ; Almeida, Andre M. / The ovine hepatic mitochondrial proteome : Understanding seasonal weight loss tolerance in two distinct breeds. In: PLoS One. 2019 ; Vol. 14, No. 2.
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Miller, BA, Chapwanya, A, Kilminster, T, Scanlon, T, Milton, J, Osorio, H, Oldham, C, Greeff, J, Bergfelt, DR, Campos, AMO & Almeida, AM 2019, 'The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds' PLoS One, vol. 14, no. 2, 0212580. https://doi.org/10.1371/journal.pone.0212580

The ovine hepatic mitochondrial proteome : Understanding seasonal weight loss tolerance in two distinct breeds. / Miller, Blake A.; Chapwanya, Aspinas; Kilminster, Tanya; Scanlon, Tim; Milton, John; Osorio, Hugo; Oldham, Chris; Greeff, Johan; Bergfelt, Don R.; Campos, Alexandre M. O.; Almeida, Andre M.

In: PLoS One, Vol. 14, No. 2, 0212580, 20.02.2019.

Research output: Contribution to journalArticle

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AU - Chapwanya, Aspinas

AU - Kilminster, Tanya

AU - Scanlon, Tim

AU - Milton, John

AU - Osorio, Hugo

AU - Oldham, Chris

AU - Greeff, Johan

AU - Bergfelt, Don R.

AU - Campos, Alexandre M. O.

AU - Almeida, Andre M.

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