The nucleotide sequence of a complementary DNA encoding Flaveria bidentis carbonic anhydrase

Antonino Cavallaro; Martha Ludwig; James Burnell

doi:10.1016/0014-5793(94)00767-5

The nucleotide sequence of a complementary DNA encoding Flaveria bidentis carbonic anhydrase

Antonino Cavallaro, Martha Ludwig, James Burnell

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

We have isolated and characterised a cDNA clone encoding the cytosolic form of carbonic anhydrase in the leaves of Flaveria bidentis, a C₄ dicotyledonous plant. The deduced amino acid sequence is similar to the carbonic anhydrase found in the chloroplasts of C₃ dicotyledonous plants. Western blot analysis of crude leaf extracts of F. bidentis indicates that the leader sequence (equivalent to the transit peptide of the chloroplastic form of CA found in C₃ plants) is not removed following translation of mRNA.

Original language	English
Pages (from-to)	216-218
Number of pages	3
Journal	FEBS Letters
Volume	350
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(94)00767-5
Publication status	Published - 22 Aug 1994
Externally published	Yes

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title = "The nucleotide sequence of a complementary DNA encoding Flaveria bidentis carbonic anhydrase",

abstract = "We have isolated and characterised a cDNA clone encoding the cytosolic form of carbonic anhydrase in the leaves of Flaveria bidentis, a C4 dicotyledonous plant. The deduced amino acid sequence is similar to the carbonic anhydrase found in the chloroplasts of C3 dicotyledonous plants. Western blot analysis of crude leaf extracts of F. bidentis indicates that the leader sequence (equivalent to the transit peptide of the chloroplastic form of CA found in C3 plants) is not removed following translation of mRNA.",

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author = "Antonino Cavallaro and Martha Ludwig and James Burnell",

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T1 - The nucleotide sequence of a complementary DNA encoding Flaveria bidentis carbonic anhydrase

AU - Cavallaro, Antonino

AU - Ludwig, Martha

AU - Burnell, James

PY - 1994/8/22

Y1 - 1994/8/22

N2 - We have isolated and characterised a cDNA clone encoding the cytosolic form of carbonic anhydrase in the leaves of Flaveria bidentis, a C4 dicotyledonous plant. The deduced amino acid sequence is similar to the carbonic anhydrase found in the chloroplasts of C3 dicotyledonous plants. Western blot analysis of crude leaf extracts of F. bidentis indicates that the leader sequence (equivalent to the transit peptide of the chloroplastic form of CA found in C3 plants) is not removed following translation of mRNA.

AB - We have isolated and characterised a cDNA clone encoding the cytosolic form of carbonic anhydrase in the leaves of Flaveria bidentis, a C4 dicotyledonous plant. The deduced amino acid sequence is similar to the carbonic anhydrase found in the chloroplasts of C3 dicotyledonous plants. Western blot analysis of crude leaf extracts of F. bidentis indicates that the leader sequence (equivalent to the transit peptide of the chloroplastic form of CA found in C3 plants) is not removed following translation of mRNA.

KW - C plant

KW - Carbonic anhydrase

KW - Flaveria bidentis

KW - Leader sequence

KW - Transit peptide

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DO - 10.1016/0014-5793(94)00767-5

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VL - 350

SP - 216

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JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -

The nucleotide sequence of a complementary DNA encoding Flaveria bidentis carbonic anhydrase

Abstract

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