The murine choline-deficient, ethionine-supplemented (CDE) diet model of chronic liver injury

Jully Gogoi-Tiwari, Julia Köhn-Gaone, Corey Giles, Dirk Schmidt-Arras, Francis D. Gratte, Caryn L. Elsegood, Geoffrey W. McCaughan, Grant A. Ramm, John K. Olynyk, Janina E.E. Tirnitz-Parker

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Chronic liver diseases, such as viral hepatitis, alcoholic liver disease, or non-alcoholic fatty liver disease, are characterized by continual inflammation, progressive destruction and regeneration of the hepatic parenchyma, liver progenitor cell proliferation, and fibrosis. The end-stage of every chronic liver disease is cirrhosis, a major risk factor for the development of hepatocellular carcinoma. To study processes regulating disease initiation, establishment, and progression, several animal models are used in laboratories. Here we describe a six-week time course of the choline-deficient and ethionine-supplemented (CDE) mouse model, which involves feeding six-week old male C57BL/6J mice with choline-deficient chow and 0.15% DL-ethionine-supplemented drinking water. Monitoring of animal health and a typical body weight loss curve are explained. The protocol demonstrates the gross examination of a CDE-treated liver and blood collection by cardiac puncture for subsequent serum analyses. Next, the liver perfusion technique and collection of different hepatic lobes for standard evaluations are shown, including liver histology assessments by hematoxylin and eosin or Sirius Red stainings, immunofluorescent detection of hepatic cell populations as well as transcriptome profiling of the liver microenvironment. This mouse model is suitable for studying inflammatory, fibrogenic, and liver progenitor cell dynamics induced through chronic liver disease and can be used to test potential therapeutic agents that may modulate these processes.

Original languageEnglish
Article numbere56138
JournalJournal of Visualized Experiments
Volume2017
Issue number128
DOIs
Publication statusPublished - 21 Oct 2017

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Ethionine
Nutrition
Choline
Liver
Diet
Wounds and Injuries
Liver Diseases
Chronic Disease
Fibrosis
Stem Cells
Alcoholic Liver Diseases
Animals
Gene Expression Profiling
Hematoxylin
Eosine Yellowish-(YS)
Inbred C57BL Mouse
Punctures
Drinking Water
Hepatitis
Histology

Cite this

Gogoi-Tiwari, Jully ; Köhn-Gaone, Julia ; Giles, Corey ; Schmidt-Arras, Dirk ; Gratte, Francis D. ; Elsegood, Caryn L. ; McCaughan, Geoffrey W. ; Ramm, Grant A. ; Olynyk, John K. ; Tirnitz-Parker, Janina E.E. / The murine choline-deficient, ethionine-supplemented (CDE) diet model of chronic liver injury. In: Journal of Visualized Experiments. 2017 ; Vol. 2017, No. 128.
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abstract = "Chronic liver diseases, such as viral hepatitis, alcoholic liver disease, or non-alcoholic fatty liver disease, are characterized by continual inflammation, progressive destruction and regeneration of the hepatic parenchyma, liver progenitor cell proliferation, and fibrosis. The end-stage of every chronic liver disease is cirrhosis, a major risk factor for the development of hepatocellular carcinoma. To study processes regulating disease initiation, establishment, and progression, several animal models are used in laboratories. Here we describe a six-week time course of the choline-deficient and ethionine-supplemented (CDE) mouse model, which involves feeding six-week old male C57BL/6J mice with choline-deficient chow and 0.15{\%} DL-ethionine-supplemented drinking water. Monitoring of animal health and a typical body weight loss curve are explained. The protocol demonstrates the gross examination of a CDE-treated liver and blood collection by cardiac puncture for subsequent serum analyses. Next, the liver perfusion technique and collection of different hepatic lobes for standard evaluations are shown, including liver histology assessments by hematoxylin and eosin or Sirius Red stainings, immunofluorescent detection of hepatic cell populations as well as transcriptome profiling of the liver microenvironment. This mouse model is suitable for studying inflammatory, fibrogenic, and liver progenitor cell dynamics induced through chronic liver disease and can be used to test potential therapeutic agents that may modulate these processes.",
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Gogoi-Tiwari, J, Köhn-Gaone, J, Giles, C, Schmidt-Arras, D, Gratte, FD, Elsegood, CL, McCaughan, GW, Ramm, GA, Olynyk, JK & Tirnitz-Parker, JEE 2017, 'The murine choline-deficient, ethionine-supplemented (CDE) diet model of chronic liver injury' Journal of Visualized Experiments, vol. 2017, no. 128, e56138. https://doi.org/10.3791/56138

The murine choline-deficient, ethionine-supplemented (CDE) diet model of chronic liver injury. / Gogoi-Tiwari, Jully; Köhn-Gaone, Julia; Giles, Corey; Schmidt-Arras, Dirk; Gratte, Francis D.; Elsegood, Caryn L.; McCaughan, Geoffrey W.; Ramm, Grant A.; Olynyk, John K.; Tirnitz-Parker, Janina E.E.

In: Journal of Visualized Experiments, Vol. 2017, No. 128, e56138, 21.10.2017.

Research output: Contribution to journalArticle

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AU - Gogoi-Tiwari, Jully

AU - Köhn-Gaone, Julia

AU - Giles, Corey

AU - Schmidt-Arras, Dirk

AU - Gratte, Francis D.

AU - Elsegood, Caryn L.

AU - McCaughan, Geoffrey W.

AU - Ramm, Grant A.

AU - Olynyk, John K.

AU - Tirnitz-Parker, Janina E.E.

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KW - Choline-deficient

KW - Chronic liver disease

KW - Ethionine-supplemented diet

KW - Fibrosis

KW - Histology

KW - Inflammatory response

KW - Issue 128

KW - Liver isolation

KW - Liver microenvironment

KW - Liver perfusion

KW - Liver progenitor cells

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