Abstract
In this thesis an efficient protocol for the Agrobacterium-mediated transient transformation of the
leaves of the C4 model organism Flaveria bidentis is presented. This technique was utilised to
investigate the transcriptional regulation of two key C4-associated genes in Flaveria;
phosphoenolpyruvate carboxylase (ppcA1) and carbonic anhydrase (βca3a). Additionally, the
stepwise molecular evolution of βca3a was inferred through the analysis of carbonic anhydrase
genes from multiple Flaveria species. Collectively, this thesis provides a novel method for the
investigation of, and new insights into, the molecular evolution of C4 photosynthesis in the genus
Flaveria.
leaves of the C4 model organism Flaveria bidentis is presented. This technique was utilised to
investigate the transcriptional regulation of two key C4-associated genes in Flaveria;
phosphoenolpyruvate carboxylase (ppcA1) and carbonic anhydrase (βca3a). Additionally, the
stepwise molecular evolution of βca3a was inferred through the analysis of carbonic anhydrase
genes from multiple Flaveria species. Collectively, this thesis provides a novel method for the
investigation of, and new insights into, the molecular evolution of C4 photosynthesis in the genus
Flaveria.
Original language | English |
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Qualification | Doctor of Philosophy |
Awarding Institution |
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Supervisors/Advisors |
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Award date | 16 Nov 2023 |
DOIs | |
Publication status | Unpublished - 2023 |